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Titolo:
SKELETAL MYOBLAST TRANSPLANTATION FOR REPAIR OF MYOCARDIAL NECROSIS
Autore:
MURRY CE; WISEMAN RW; SCHWARTZ SM; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,SCH MED,DEPT PATHOL,BOX 357470,ROOM E-520,HSB SEATTLEWA 98195 UNIV WASHINGTON,SCH MED,DEPT RADIOL SEATTLE WA 98195 UNIV WASHINGTON,SCH MED,DEPT BIOCHEM SEATTLE WA 98195
Titolo Testata:
The Journal of clinical investigation
fascicolo: 11, volume: 98, anno: 1996,
pagine: 2512 - 2523
SICI:
0021-9738(1996)98:11<2512:SMTFRO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SATELLITE CELL IMPLANTATION; NUCLEAR MITOTIC DIVISION; DNA-SYNTHESIS; MUSCLE DIFFERENTIATION; GENE-EXPRESSION; RAT MYOCARDIUM; N-CADHERIN; REGENERATION; GRAFTS; MYOGENESIS;
Keywords:
MYOCARDIAL INFARCTION; SKELETAL MYOBLAST; MYOSIN HEAVY CHAIN; CONTRACTILE FUNCTION; CELL TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
C.E. Murry et al., "SKELETAL MYOBLAST TRANSPLANTATION FOR REPAIR OF MYOCARDIAL NECROSIS", The Journal of clinical investigation, 98(11), 1996, pp. 2512-2523

Abstract

Myocardial infarcts heal by scarring because myocardium cannot regenerate. To determine if skeletal myoblasts could establish new contractile tissue, hearts of adult inbred rats were injured by freeze-thaw, and 3-4.5 x 10(6) neonatal skeletal muscle cells were transplanted immediately thereafter. At 1 d the graft cells were proliferating and did not express myosin heavy chain (MHC). By 3 d, multinucleated myotubes were present which expressed both embryonic and fast fiber MHCs. At 2 wk, electron microscopy demonstrated possible satellite stem cells. By 7 wk the grafts began expressing beta-MHC, a hallmark of the slow fiber phenotype; coexpression of embryonic, fast, and beta-MHC continued through 3 mo. Transplanting myoblasts 1 wk after injury yielded comparable results, except that grafts expressed beta-MHC sooner (by 2 wk). Grafts never expressed cardiac-specific MHC-alpha. Wounds containing 2-wk-old myoblast grafts contracted when stimulated ex vivo, and high frequency stimulation induced tetanus. Furthermore, the grafts could perform a cardiac-like duty cycle, alternating tetanus and relaxation, for at least 6 min. Thus, skeletal myoblasts can establish new muscle tissue when grafted into injured hearts, and this muscle can contract when stimulated electrically. Because the grafts convert to fatigue-resistant, slow twitch fibers, this new muscle may be suited to a cardiac work load.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:36:06