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Titolo:
FUNCTIONAL-STUDIES OF RAT, PORCINE, AND HUMAN PANCREATIC-ISLETS CULTURED IN 10 COMMERCIALLY AVAILABLE MEDIA
Autore:
HOLMES MA; CLAYTON HA; CHADWICK DR; BELL PRF; LONDON NJM; JAMES RFL;
Indirizzi:
UNIV LEICESTER,ROYAL INFIRM,DEPT SURG,CLIN SCI BLDG LEICESTER LE2 7LXLEICS ENGLAND UNIV LEICESTER,ROYAL INFIRM,DEPT SURG LEICESTER LE2 7LX LEICS ENGLAND
Titolo Testata:
Transplantation
fascicolo: 8, volume: 60, anno: 1995,
pagine: 854 - 860
SICI:
0041-1337(1995)60:8<854:FORPAH>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM-FREE MEDIUM; ADULT-PIG ISLETS; CELLS; INSULIN; NICOTINAMIDE; INVITRO; PURIFICATION; LANGERHANS; INHIBITION; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Holmes et al., "FUNCTIONAL-STUDIES OF RAT, PORCINE, AND HUMAN PANCREATIC-ISLETS CULTURED IN 10 COMMERCIALLY AVAILABLE MEDIA", Transplantation, 60(8), 1995, pp. 854-860

Abstract

There have been no extensive studies investigating the effect of tissue culture media on the in vitro functional characteristics of rat, porcine and human Islets of Langerhans. We therefore aimed to compare ten commercially available tissue culture media on the basis of their ability to maintain islet viability. Following isolation, islets were cultured free-floating in the ten media (RPMI 1640-11mM glucose (control), RPMI 1640-2.2mM glucose, Dulbecco's MEM, TCM 199, CMRL 1066, Iscove's MEM, Waymouth's MEM, Serum-Free medium, Ex-cell 300, Ham's F-12) and viability was assessed after 24 hr, 3 days, and 7 days on the basis of macroscopic appearance, cell membrane integrity, and insulin secretion in response to glucose stimulation both by dynamic incubation and by perifusion. Each islet species demonstrated physiological insulin release characteristics in all media-however, it was possible to distinguish between the media by comparing the stimulation indices calculated from the insulin release studies. Significantly higher stimulation indices were produced in Iscove's MEM for rat islets, in Ham's F-12 forporcine islets and in CMRL 1066 for human islets. Over the entire culture period a significant deterioration in function was observed in all species cultured in the control media, although this was reversed when islets were cultured in the optimal media. Furthermore, in the caseof porcine and human islets a significant improvement in function over the seven-day period was noted in the optimal media. In conclusion, of the commercially available media, the optimal tissue culture mediumfor rat islets is Iscove's MEM, for porcine islets is Ham's F-12, andfor human islets is CMRL 1066.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:09:15