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Titolo:
REGULATION OF OXYTOCIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN THE VENTROMEDIAL HYPOTHALAMUS BY TESTOSTERONE AND ITS METABOLITES
Autore:
BALE TL; DORSA DM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,BOX 357280 SEATTLE WA 98195 UNIV WASHINGTON,DEPT PSYCHIAT & BEHAV SCI SEATTLE WA 98195
Titolo Testata:
Endocrinology
fascicolo: 11, volume: 136, anno: 1995,
pagine: 5135 - 5138
SICI:
0013-7227(1995)136:11<5135:ROORMI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT MICROSCOPIC AUTORADIOGRAPHY; RAT-BRAIN; BINDING-SITES; MATERNAL-BEHAVIOR; FEMALE RATS; GONADAL-STEROIDS; ESTROGEN; NUCLEUS; LOCALIZATION; VASOPRESSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
T.L. Bale e D.M. Dorsa, "REGULATION OF OXYTOCIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN THE VENTROMEDIAL HYPOTHALAMUS BY TESTOSTERONE AND ITS METABOLITES", Endocrinology, 136(11), 1995, pp. 5135-5138

Abstract

Oxytocin receptor (OR) binding in the ventromedial hypothalamus (VMH)is regulated by testosterone (T) and its metabolites, estrogen (E(2))and dihydrotestosterone (DHT). Previous studies have reported that ORbinding increases in the VMH in castrated male rats when they are replaced with T or E(2) compared to that in vehicle-treated animals. DHT alone had no effect on OR binding, but when given in combination with E(2) appeared to have a synergistic effect. This study was designed todetermine whether these effects of steroid hormones on OR binding in the VMH are associated with changes in OR messenger RNA (mRNA) expression. Male rats were castrated or sham operated and given T propionate (TP), E(2) benzoate (EB), DHT plus EB, or an oil vehicle. OR mRNA was assessed using a rat complementary RNA OR probe and in situ hybridization techniques. OR binding to tissue slices was quantified autoradiographically using an OR antagonist, -125]d(CH2)(5)[Tyr(Me)(2)Thr(4),Tyr-NH29]ornithine vasotocin. These experiments showed that TP and EB increased both OR mRNA and OR binding in the VMH significantly above levels in vehicle-treated animals. However, animals given both EB and DHT exhibited significantly lower OR mRNA expression and OR binding in the VMH compared to those in animals treated with TP or EB alone. These data indicate that increases in VMH OR binding in response to gonadal steroids are accompanied by changes at the mRNA level that correspond well in magnitude and direction with those in the OR-binding sites.

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Documento generato il 30/03/20 alle ore 19:20:00