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Titolo:
SUSCEPTIBILITY OF EXOGENOUS SURFACTANT TO PHOSPHOLIPASE A(2) DEGRADATION
Autore:
DUNCAN JE; HATCH GM; BELIK J;
Indirizzi:
FOOTHILLS PROV GEN HOSP,ROOM C211,1403 29TH ST NW CALGARY AB T2N 2T9 CANADA UNIV MANITOBA,FAC MED,DEPT PHYSIOL WINNIPEG MB R3E 0L8 CANADA UNIV MANITOBA,FAC MED,DEPT INTERNAL MED WINNIPEG MB R3E 0L8 CANADA UNIV MANITOBA,FAC MED,DEPT BIOCHEM & MOL BIOL WINNIPEG MB R3E 0L8 CANADA UNIV MANITOBA,FAC MED,DEPT PEDIAT WINNIPEG MB R3E 0L8 CANADA
Titolo Testata:
Canadian journal of physiology and pharmacology
fascicolo: 8, volume: 74, anno: 1996,
pagine: 957 - 963
SICI:
0008-4212(1996)74:8<957:SOESTP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS SYNDROME; PULMONARY SURFACTANT; SYNTHETIC SURFACTANT; SEPTIC SHOCK; LUNG; PURIFICATION; DISEASE; PHOSPHATIDYLCHOLINE; INHIBITION; SECRETION;
Keywords:
DIPALMITOYLPHOSPHATIDYLCHOLINE; TYLOXAPOL; SURFACTANT INACTIVATION; EXOSURF(R); SURVANTA(R); PHOSPHOLIPASE A(2) INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.E. Duncan et al., "SUSCEPTIBILITY OF EXOGENOUS SURFACTANT TO PHOSPHOLIPASE A(2) DEGRADATION", Canadian journal of physiology and pharmacology, 74(8), 1996, pp. 957-963

Abstract

The inhibition of surfactant biophysical activity in vivo is potentially mediated by many factors, including serum proteins, particularly enzymatic proteins such as phospholipases. In the present study, we investigated the susceptibility of the phosphatidylcholine component of two exogenous surfactants, Exosurf(R) and Survanta(R), to secretory-type phospholipase A(2) (PLA(2)) deacylation in vitro. Lyophilized Exosurf and Survanta preparations were incubated at 37 degrees C for 120 minin the presence of bovine pancreatic PLA(2), and the production of lysophosphatidylcholine was determined as a measure of the magnitude of phosphatidylcholine deacylation. The phosphatidylcholine component of Survanta was readily deacylated by PLA(2), whereas the dipalmitoylphosphatidycholine (DPPC) component of Exosurf was resistant over the entire duration of the assay. To further evaluate this observed resistancethe individual and combined effects of tyloxapol and hexadecanol, components of Exosurf, upon PLA(2) deacylation of Survanta and DPPC were investigated. In both Survanta and DPPC preparations, PLA(2)-mediated deacylation was significantly inhibited in the presence of tyloxapol. We conclude that the presence of tyloxapol in the Exosurf preparation inhibits secretory type PLA(2) mediated DPPC deacylation. This unique feature of Exosurf may be of clinical significance when this preparation is utilized in the treatment of surfactant-deficient infants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:17:08