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Titolo:
EXTENSIVE ALTERNATE EXON USAGE AT THE 5'-END OF THE SHEEP TROPOELASTIN GENE
Autore:
MAUCH JC; SANDBERG LB; ROOS PJ; JIMENEZ F; CHRISTIANO AM; DEAK SB; BOYD CD;
Indirizzi:
LOMA LINDA UNIV,PETTIS VA HOSP,DEPT MED LOMA LINDA CA 92357 LOMA LINDA UNIV,PETTIS VA HOSP,DEPT PATHOL LOMA LINDA CA 92357 THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT DERMATOL PHILADELPHIA PA 19107 UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT SURG NEW BRUNSWICK NJ 08903
Titolo Testata:
Matrix biology
fascicolo: 8, volume: 14, anno: 1995,
pagine: 635 - 641
SICI:
0945-053X(1995)14:8<635:EAEUAT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; ELASTIN GENE; PROTEIN; HETEROGENEITY; SEQUENCE; DISEASE; REGION;
Keywords:
ALTERNATIVE SPLICING; POLYMERASE CHAIN REACTION; TROPOELASTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Mauch et al., "EXTENSIVE ALTERNATE EXON USAGE AT THE 5'-END OF THE SHEEP TROPOELASTIN GENE", Matrix biology, 14(8), 1995, pp. 635-641

Abstract

Several overlapping cDNA clones were isolated from a lambda gtl0 cDNAlibrary constructed using polyA+ RNA from neonatal sheep lung. DNA sequence analysis of these cDNA recombinants revealed the complete derived amino acid sequence of sheep tropoelastin. A comparison of DNA sequences from individual sheep tropoelastin cDNA also confirmed the presence of several tropoelastin mRNA isoforms in neonatal lung tissue. Coding domains corresponding to exons 13, 14 and 33 were present in several of the sheep tropoelastin cDNA fragments but absent in others. The relative amount of alternate usage of these exons was quantitated by polymerase chain amplification. In confirmation of previous studies in other mammalian species, extensive alternate usage of exon 33 was observed in total RNA isolated from aorta, nuchal ligament and pulmonary artery from neonatal sheep. In striking contrast to all previous studies, however, exons 13 and 14 were shown to be subject to almost the same level of alternate usage as exon 33 in all three neonatal sheep tissues examined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:44:56