Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
2-DIMENSIONAL GEL-ELECTROPHORESIS ANALYSIS OF THE RESPONSE OF PSEUDOMONAS-PUTIDA KT2442 TO 2-CHLOROPHENOL
Autore:
LUPI CG; COLANGELO T; MASON CA;
Indirizzi:
SWISS FED INST ENVIRONM SCI & TECHNOL,UEBERLANDSTR 133 CH-8600 DUBENDORF SWITZERLAND SWISS FED INST ENVIRONM SCI & TECHNOL CH-8600 DUBENDORF SWITZERLAND
Titolo Testata:
Applied and environmental microbiology
fascicolo: 8, volume: 61, anno: 1995,
pagine: 2863 - 2872
SICI:
0099-2240(1995)61:8<2863:2GAOTR>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMOBILIZED PH GRADIENTS; UNIVERSAL STRESS PROTEIN; ESCHERICHIA-COLI; HEAT-SHOCK; POLLUTANT STRESS; GENE-EXPRESSION; CURRENT STATE; STARVATION; BACTERIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
C.G. Lupi et al., "2-DIMENSIONAL GEL-ELECTROPHORESIS ANALYSIS OF THE RESPONSE OF PSEUDOMONAS-PUTIDA KT2442 TO 2-CHLOROPHENOL", Applied and environmental microbiology, 61(8), 1995, pp. 2863-2872

Abstract

The effects of exposure of Pseudomonas putida KT2442 to 2-chlorophenol as a model for the chemical stress response were examined by two-dimensional polyacrylamide gel electrophoresis, Individual protein concentrations were determined at 45, 65, and 95 min following the addition of 2-chlorophenol at a concentration of 1.63 mM to exponentially growing cultures of P. putida KT2442 by silver staining the separated proteins, The changes in the protein concentrations could be classified into four categories, namely those which increased continuously during exposure, those which decreased in concentration, those which showed a concentration peak at some point following exposure, and those which were essentially unaffected. Thirty proteins with isoelectric points between pH 4 and 6 increased in concentration, 27 decreased, and 90 had aconcentration maximum or minimum between 45 and 95 min, Of those proteins with isoelectric points between 5.5 and 10, 68 increased in concentration, 39 decreased in concentration, and 47 showed a concentrationpeak in the middle of the sampling period, Thus, in the evaluation ofthe stress response, a functional description requires an understanding both of proteins which are required at higher concentrations and ofthose whose presence appears to be no longer essential.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 19:22:40