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Titolo:
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PORPHYROMONAS-GINGIVALIS FIMBRIAE
Autore:
SOJAR HT; HAMADA N; GENCO RJ;
Indirizzi:
SUNY BUFFALO,SCH DENT MED,DEPT ORAL BIOL,FOSTER HALL,3435 MAIN ST BUFFALO NY 14214 SUNY BUFFALO,SCH MED & BIOMED SCI,DEPT MICROBIOL BUFFALO NY 14214
Titolo Testata:
Protein expression and purification
fascicolo: 1, volume: 9, anno: 1997,
pagine: 49 - 52
SICI:
1046-5928(1997)9:1<49:HLSOP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANAEROBE BACTEROIDES-GINGIVALIS; PURIFICATION; MELANINOGENICUS; PROTEIN; ASACCHAROLYTICUS; PORPHYROMONAS-(BACTEROIDES)-GINGIVALIS; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
H.T. Sojar et al., "HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PORPHYROMONAS-GINGIVALIS FIMBRIAE", Protein expression and purification, 9(1), 1997, pp. 49-52

Abstract

Fimbriae are responsible for the adherence of many bacterial species to the surfaces they eventually colonize. Porphyromonas gingivalis is an important pathogenic agent involved in periodontal disease. Fimbriae of P.gingivalis have been thought to mediate binding of the bacterium to oral surfaces. In order to study the role of fimbriae in microbial adhesion, it is important to purify fimbriae to homogeneity. A simple and rapid reverse-phase high-performance liquid chromatography (HPLC) method is developed to purify P. gingivalis fimbriae. The crude fimbriae were precipitated from sonic extract of P. gingivalis cells with the 40% ammonium sulfate precipitation. The dialyzed crude fimbriae preparation was subjected to reverse-phase HPLC separation. The purity and size of purified fimbrial proteins were confirmed by SDS-polyacrylamide gel electrophoresis(SDS-PAGE) followed by Western immunoblot using polyclonal antibodies against fimbriae. The purified major fimbrial protein from strain 33277 of P. gingivalis had apparent molecular massof 41 kDa. The method is useful for analytical as well as preparativepurification with 25% yields from the ammonium sulfate-precipitated crude fimbriae preparation, and represents increased speed and efficiency over earlier published methods. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:38:58