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Titolo:
ACTIVATION OF P44 AND P42 MAP KINASES IS NOT ESSENTIAL FOR THE SURVIVAL OF RAT SYMPATHETIC NEURONS
Autore:
VIRDEE K; TOLKOVSKY AM;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND UNIV CAMBRIDGE,DEPT BIOCHEM CAMBRIDGE CB2 1QW ENGLAND
Titolo Testata:
European journal of neuroscience
fascicolo: 10, volume: 7, anno: 1995,
pagine: 2159 - 2169
SICI:
0953-816X(1995)7:10<2159:AOPAPM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; SIGNAL TRANSDUCING RECEPTOR; CILIARY NEUROTROPHIC FACTOR; DEPENDENT PROTEIN-KINASE; PC12 CELLS; CYCLIC-AMP; NEURITE OUTGROWTH; DIFFERENTIATION; PHOSPHORYLATION; PATHWAY;
Keywords:
CELL DEATH; APOPTOSIS; RAS; NGF; CNTF; LIF; CYCLIC AMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
K. Virdee e A.M. Tolkovsky, "ACTIVATION OF P44 AND P42 MAP KINASES IS NOT ESSENTIAL FOR THE SURVIVAL OF RAT SYMPATHETIC NEURONS", European journal of neuroscience, 7(10), 1995, pp. 2159-2169

Abstract

We have examined whether activation of MAP kinases [or extracellular signal-regulated kinases (ERKs)] is required for the survival of rat sympathetic neurons by comparing the actions of three survival factors whose survival-promoting actions can be blocked by neutralizing Fab fragments to p21ras (Nobes and Tolkovsky, 1995, Eur. J. Neurosci., 7, 344-350), nerve growth factor (NGF), the cytokines ciliary neurotrophic factor (CNTF) and leukaemia inhibitory factor (LIF), and the cyclic AMP analogue 4-(8-chlorophenylthio)cAMP (CPTcAMP). NGF-induced survival was accompanied by an intense (15- to 30-fold) and steady (>24 h) activation of p44 and p42 ERKs which waned rapidly (t(1/2) similar to 30 min) upon NGF withdrawal. However, concentrations of NGF that induced aweak (4- to 5-fold) stimulation of the ERKs were not sufficient to maintain long-term survival. Moreover, prolonged and intense stimulationof the ERKs by NGF for up to 15.5 h was unable to confer long-term survival, since withdrawal of NGF after this time resulted in neuronal death that was kinetically indistinguishable from the death of neurons that had not been exposed to NGF. By contrast, CNTF and LIF continued to support survival for up to 3 days after eliciting only transient (<30 min and 1 h respectively) activation of p44 and p42 ERKs, while CPTcAMP induced survival for several days without any measurable activation of the ERKs, Taken together, these data suggest that ERK activationper se is neither necessary nor sufficient for survival and that alternative pathways exist for effecting long-term survival of rat sympathetic neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:19:29