Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PILUS BIOGENESIS AND EPITHELIAL-CELL ADHERENCE OF NEISSERIA-GONORRHOEAE PILC DOUBLE KNOCK-OUT MUTANTS
Autore:
RUDEL T; BOXBERGER HJ; MEYER TF;
Indirizzi:
MAX PLANCK INST BIOL,INFEKT BIOL ABT,SPEMANNSTR 34 D-72076 TUBINGEN GERMANY MAX PLANCK INST BIOL,INFEKT BIOL ABT D-72076 TUBINGEN GERMANY MAX PLANCK INST INFEKT BIOL D-10117 BERLIN GERMANY
Titolo Testata:
Molecular microbiology
fascicolo: 6, volume: 17, anno: 1995,
pagine: 1057 - 1071
SICI:
0950-382X(1995)17:6<1057:PBAEAO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGENIC VARIATION; GENE CONVERSION; PSEUDOMONAS-AERUGINOSA; GONOCOCCUS INFECTION; ESCHERICHIA-COLI; TYPE-4 FIMBRIAE; PHASE VARIATION; DNA-SEQUENCE; ADHESION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
T. Rudel et al., "PILUS BIOGENESIS AND EPITHELIAL-CELL ADHERENCE OF NEISSERIA-GONORRHOEAE PILC DOUBLE KNOCK-OUT MUTANTS", Molecular microbiology, 17(6), 1995, pp. 1057-1071

Abstract

The phase-variable PilC protein of pathogenic Neisseria species have recently been implicated in both assembly and cellular adherence functions of the type 4 pili of these pathogens. We describe here the cloning of full-length pilC1 and pilC2 genes and the complete sequencing ofthe pilC2 gene of Neisseria gonorrhoeae MS11. Sequential inactivationof both genes by gene replacement in piliated (P+) variants of N. gonorrhoeae MS11 led initially to a non-piliated (P-) phenotype; however,spontaneous P+ variants could be derived from some pilC1,2 double mutants which produced morphologically intact pili. Purified pili from pilC1,2 mutants revealed no detectable PilC protein. Instead, a novel protein about 70 kDa in size appeared in pili preparations of P+ mutants; this protein exhibited no immunological cross-reactivity with PilC1 or PilC2. We propose that this novel factor replaces the function of PilC in pilus biogenesis. Using isogenic N. gonorrhoeae strains which produce identical PilE (pilin) proteins we demonstrate that pili associated with the 70 kDa protein do not confer gonococcal adherence to human epithelial cells, in contrast to pili assembled in the presence of PilC1 or PilC2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:07:21