Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ALTERED LIGNIN COMPOSITION IN TRANSGENIC TOBACCO EXPRESSING O-METHYLTRANSFERASE SEQUENCES IN SENSE AND ANTISENSE ORIENTATION
Autore:
ATANASSOVA R; FAVET N; MARTZ F; CHABBERT B; TOLLIER MT; MONTIES B; FRITIG B; LEGRAND M;
Indirizzi:
UNIV STRASBOURG 1,CNRS,INST BIOL MOLEC PLANTES,12 RUE GEN ZIMMER F-67084 STRASBOURG FRANCE INRA,INA PG,CHIM BIOL LAB F-78850 THIVERVAL GRIGNON FRANCE
Titolo Testata:
Plant journal
fascicolo: 4, volume: 8, anno: 1995,
pagine: 465 - 477
SICI:
0960-7412(1995)8:4<465:ALCITT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSYL-L-METHIONINE; CAFFEOYL-COA 3-O-METHYLTRANSFERASE; CELL-SUSPENSION CULTURES; CHALCONE SYNTHASE GENE; DISEASE RESISTANCE; BROWN-MIDRIB; MOLECULAR-CLONING; FLOWER PIGMENTATION; PHENOLIC-COMPOUNDS; MOSAIC-VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
R. Atanassova et al., "ALTERED LIGNIN COMPOSITION IN TRANSGENIC TOBACCO EXPRESSING O-METHYLTRANSFERASE SEQUENCES IN SENSE AND ANTISENSE ORIENTATION", Plant journal, 8(4), 1995, pp. 465-477

Abstract

The monomeric composition of tobacco lignin has been modified by genetic engineering. Sense or antisense expression of sequences encoding O-methyltransferase (OMT), a lignin biosynthetic enzyme, was shown to modulate enzyme activity. Ten constructs harboring the entire or a partial OMT cDNA were used. Populations of 20 transgenic plants per construct were analyzed for OMT activity and compared with untransformed controls. As expected, expression of only the full-length sense constructled to an increase in OMT activity. An important reduction of activity was found in a variable number of plantlets from all other transgenic populations but the inhibition was sustained through the adult stageonly in plants transformed with the complete cDNA. T-DNA genes were shown to be stably integrated into the tobacco genome and to be transmitted to the progeny. By using gene-specific probes, OMT inhibition in stems was correlated to a parallel disappearance of OMT transcripts originating from both the resident gene and the transgene. In contrast, transgene transcripts were detected in leaf tissues where the residentgene is poorly expressed, thus indicating that relative expression ofthe two OMT genes controls transcript turnover. In stems of inhibitedplants, a marked decrease of syringyl units and the appearance of 5-hydroxy guaiacyl units were demonstrated. These two structural featuresare also characteristic of natural mutants of maize with an improved digestibility compared with wild lines. These data demonstrate the feasibility and the potential benefits of lignin manipulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:54:32