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Titolo:
IMMUNOPHENOTYPIC ANALYSIS OF FELINE HEMOLYMPHATIC NEOPLASIA USING FLOW-CYTOMETRY
Autore:
DEAN GA; GROSHEK PM; JAIN NC; HOOVER EA;
Indirizzi:
UNIV CALIF DAVIS,SCH VET MED,DEPT MED DAVIS CA 95616 UNIV CALIF DAVIS,SCH VET MED,DEPT PATHOL DAVIS CA 95616 UNIV CALIF DAVIS,SCH VET MED,DEPT MICROBIOL DAVIS CA 95616 UNIV CALIF DAVIS,SCH VET MED,DEPT IMMUNOL DAVIS CA 95616 COLORADO STATE UNIV,COLL VET MED & BIOMED SCI,DEPT PATHOL FT COLLINS CO 80523
Titolo Testata:
Comparative haematology international
fascicolo: 2, volume: 5, anno: 1995,
pagine: 84 - 92
SICI:
0938-7714(1995)5:2<84:IAOFHN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LYMPHOBLASTIC-LEUKEMIA; CYTOCHEMICAL CHARACTERIZATION; CLASSIFICATION; CELLS; VIRUS; CAT; IDENTIFICATION; ANTIGEN; BLOOD;
Keywords:
FELINE; FLOW CYTOMETRY; IMMUNOPHENOTYPE; LEUKEMIA, MYELOPROLIFERATIVE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
G.A. Dean et al., "IMMUNOPHENOTYPIC ANALYSIS OF FELINE HEMOLYMPHATIC NEOPLASIA USING FLOW-CYTOMETRY", Comparative haematology international, 5(2), 1995, pp. 84-92

Abstract

Tissues from 17 cats with suspected haemolymphatic neoplasia were analysed using phenotype-specific monoclonal antibodies (MAbs) and flow cytometry. Eight of the cases were also classified according to the French-American-British (FAB) system for acute leukaemias and were characterised by cytochemical staining. Phenotypes identified by the panel of MAb employed here included T lymphocyte, CD4+ lymphocyte, CD8+ lymphocyte, undifferentiated lymphocyte, erythroid or myeloid. By combiningthe cytological diagnosis of neoplasia with the phenotype, a precise diagnosis was obtained in 15 of the 17 cases. Of the 15 cases successfully phenotyped, eight were T-cell, two were B-cell, one was myeloid, two were erythroid, and two were mixed erythroid and myeloid. Two samples that did not react with the panel of MAb were determined at necropsy to be of non-haemolymphatic origin. Expression of feline leukaemia virus (FeLV) by neoplastic cells was demonstrated in three of seven FeLV-positive cats. The lack of a B-lymphocyte-specific MAb was the mostsignificant deficit in the antibody panel and precluded conclusive identification of B-cell leukaemias. The FAB classification provided a more objective means to identify myelodysplastic syndrome versus myeloproliferative disorder. Cytochemical staining confirmed flow cytometricresults in non-lymphoid neoplasias but otherwise did not refine the phenotypic diagnosis made by flow cytometric analysis. In the cases presented here, immunophenotyping with flow cytometry and our panel of MAbs, accurately identified lymphoid versus non-lymphoid neoplasia, as well as single and mixed lineage myelo-proliferative disorders.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:14:47