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Titolo:
BENZENE METABOLISM IN RODENT HEPATOCYTES - ROLE OF SULFATE CONJUGATION
Autore:
ORZECHOWSKI A; SCHWARZ LR; SCHWEGLER U; BOCK KW; SNYDER R; SCHRENK D;
Indirizzi:
UNIV TUBINGEN,INST TOXICOL,WILHELMSTR 56 D-72074 TUBINGEN GERMANY UNIV TUBINGEN,INST TOXICOL D-72074 TUBINGEN GERMANY GSF MUNICH,INST TOXICOL D-85758 NEUHERBERG GERMANY EOHSI,DEPT PHARMACOL & TOXICOL PISCATAWAY NJ 08855
Titolo Testata:
Xenobiotica
fascicolo: 10, volume: 25, anno: 1995,
pagine: 1093 - 1102
SICI:
0049-8254(1995)25:10<1093:BMIRH->2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT HEPATOCYTES; TOXICITY; MICE; CARCINOGENICITY; PATHWAYS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
A. Orzechowski et al., "BENZENE METABOLISM IN RODENT HEPATOCYTES - ROLE OF SULFATE CONJUGATION", Xenobiotica, 25(10), 1995, pp. 1093-1102

Abstract

1. Hepatocytes isolated from the adult male NMRI mouse or Wistar rat were incubated for 1 h with 0.5 mM C-14-benzene, the supernatant was separated from the cells, and analysed for benzene metabolites. Separately, formation of sulphate conjugates during benzene metabolism was studied in hepatocytes in the presence of S-35-sulphate. In addition sulphate conjugation of the benzene metabolites hydroquinone and 1,2,4-trihydroxybenzene was investigated in mouse liver cytosol supplemented with 3'-phosphoadenosine-5'-phospho-(35)-sulphate. 2. Two novel metabolites, not detectable in rat hepatocyte incubations, were found in mouse hepatocytes, and were identified as 1,2,4-trihydroxybenzene sulphateand hydroquinone sulphate. Formation of the S-35-labelled conjugates could be demonstrated in incubations of mouse liver cytosol with hydroquinone or 1,2,4-trihydroxybenzene supplemented with 3'-phosphoadenosine-5'-phospho-S-35-sulphate, and in mouse hepatocytes incubated with benzene and S-35-sulphate. 3. In comparison with hepatocytes from the Wistar rat, hepatocytes from the NMRI mouse were almost three times more effective in metabolizing benzene. The higher formation of hydroquinone, and the formation of trihydroxybenzene sulphate and hydroquinone sulphate, mainly contributed to the higher rate of benzene metabolism.4. In conclusion, qualitative and quantitative differences in benzenemetabolism may contribute to the higher susceptibility of mouse towards the myelotoxic and leucaemogenic action of benzene.

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Documento generato il 22/09/20 alle ore 10:55:12