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Titolo:
URINARY THIOSULFATE DETERMINED BY SUPPRESSED ION CHROMATOGRAPHY WITH CONDUCTIMETRIC DETECTION
Autore:
COLE DEC; EVROVSKI J; PIRONE R;
Indirizzi:
UNIV TORONTO,BANTING INST,DEPT CLIN BIOCHEM,100 COLL ST,RM 415 TORONTO ON M5G 1L5 CANADA UNIV TORONTO,BANTING INST,DEPT MED TORONTO ON M5G 1L5 CANADA UNIV TORONTO,BANTING INST,DEPT PAEDIAT GENET TORONTO ON M5G 1L5 CANADA
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 1, volume: 672, anno: 1995,
pagine: 149 - 154
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW ANION CHROMATOGRAPHY; INORGANIC SULFATE; ASSAY;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
D.E.C. Cole et al., "URINARY THIOSULFATE DETERMINED BY SUPPRESSED ION CHROMATOGRAPHY WITH CONDUCTIMETRIC DETECTION", Journal of chromatography B. Biomedical applications, 672(1), 1995, pp. 149-154

Abstract

Thiosulfate is a naturally occurring product of sulfur metabolism. Assays of urinary thiosulfate have been based on the reaction with cyanide to form thiocyanate. However, matrix interferences and background variation in endogenous thiocyanate excretion place serious constraintson this method for determination of physiological amounts of thiosulfate in urine. We describe a column-switching ion chromatographic separation for urinary thiosulfate that allows for sensitive and accurate detection by ion conductimetry. In 20 adult volunteers, we found a lower urinary thiosulfate (8.50 +/- 7.39 mu mol/24 h, mean +/- S.D.) than others have described, although the upward skew of the results (median, 6.90; range, 0.84-32 mu mol/24 h) was similar. However, we have not observed any of the interferences and the sensitivity of our technique( < 0.2 mu mol/24 h) allows for detection of thiosulfate in all control samples. This sort of methodological improvement will be essential for any study of physiological thiosulfate metabolism.

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Documento generato il 04/07/20 alle ore 20:45:15