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Titolo:
BIOCOMPATIBLE SAMPLE PRETREATMENT FOR IMMUNOCHEMICAL TECHNIQUES USINGMICELLAR LIQUID-CHROMATOGRAPHY FOR SEPARATION OF CORTICOSTEROIDS
Autore:
LOVGREN U; JOHANSSON M; KRONKVIST K; EDHOLM LE;
Indirizzi:
ASTRO DRACO AB,DEPT BIOANALYT CHEM,POB 34 S-22100 LUND SWEDEN LUND UNIV,DEPT ANALYT CHEM S-22100 LUND SWEDEN
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 1, volume: 672, anno: 1995,
pagine: 33 - 44
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT SERUM INJECTION; STATIONARY PHASES; MOBILE PHASES; DRUGS; BEHAVIOR; SOLUTES; PLASMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
U. Lovgren et al., "BIOCOMPATIBLE SAMPLE PRETREATMENT FOR IMMUNOCHEMICAL TECHNIQUES USINGMICELLAR LIQUID-CHROMATOGRAPHY FOR SEPARATION OF CORTICOSTEROIDS", Journal of chromatography B. Biomedical applications, 672(1), 1995, pp. 33-44

Abstract

Micellar liquid chromatography (MLC) using Tween 20 as surfactant wasevaluated as a biocompatible sample pretreatment preceding immunoassay in order to obtain an increased selectivity of the assay and a simplification of the sample pretreatment procedure. Different stationary phases and chromatographic conditions were studied for the separation of budesonide and cortisol and some steroids known to interfere in immunoassay of these compounds. The separation was dependent on several parameters, for example, temperature, the concentration of Tween 20, pH and ionic strength of the mobile phase, and nature of the stationary phase. A precolumn venting system was used, which allowed for 140 direct injections of 25 mu l of human blood plasma, without loss of chromatographic performance. Results obtained from the coupling of MLC to an immunoassay for cortisol illustrates the selectivity which can be obtained, and that simplification of the sample pretreatment is possible using this technique.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 00:20:24