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Titolo:
NUMA IS REQUIRED FOR THE ORGANIZATION OF MICROTUBULES INTO ASTER-LIKEMITOTIC ARRAYS
Autore:
GAGLIO T; SAREDI A; COMPTON DA;
Indirizzi:
DARTMOUTH COLL SCH MED,DEPT BIOCHEM HANOVER NH 03755
Titolo Testata:
The Journal of cell biology
fascicolo: 3, volume: 131, anno: 1995,
pagine: 693 - 708
SICI:
0021-9525(1995)131:3<693:NIRFTO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; CYTOPLASMIC DYNEIN; APPARATUS PROTEIN; SPINDLE FORMATION; NUCLEAR-ENVELOPE; SPN ANTIGEN; PTK2 CELLS; MITOSIS; END; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
T. Gaglio et al., "NUMA IS REQUIRED FOR THE ORGANIZATION OF MICROTUBULES INTO ASTER-LIKEMITOTIC ARRAYS", The Journal of cell biology, 131(3), 1995, pp. 693-708

Abstract

NuMA (Nuclear protein that associates with the Mitotic Apparatus) is a 235-kD intranuclear protein that accumulates at the pericentrosomal region of the mitotic spindle in vertebrate cells. To determine if NuMA plays an active role in organizing the microtubules at the polar region of the mitotic spindle, we have developed a cell free system for the assembly of mitotic asters derived from synchronized cultured cells. Mitotic asters assembled in this extract are composed of microtubules arranged in a radial array that contain NuMA concentrated at the central core. The organization of microtubules into asters in this cell free system is dependent on NuMA because immunodepletion of NuMA from the extract results in randomly dispersed microtubules instead of organized mitotic asters, and addition of the purified recombinant NuMA protein to the NuMA-depleted extract fully reconstitutes the organizationof the microtubules into mitotic asters. Furthermore, we show that NuMA is phosphorylated upon mitotic aster assembly and that NuMA is onlyrequired in the late stages of aster assembly in this cell free system consistent with the temporal accumulation of NuMA at the polar ends of the mitotic spindle in vivo. These results, in combination with thephenotype observed in vivo after the prevention of NuMA from targeting onto the mitotic spindle by antibody microinjection, suggest that NuMA plays a functional role in the organization of the microtubules of the mitotic spindle.

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Documento generato il 25/11/20 alle ore 00:42:19