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Titolo:
CHRONIC ORAL-ADMINISTRATION OF SYNTHETIC TRYPSIN-INHIBITOR CAMOSTATE REDUCES AMYLASE RELEASE FROM ISOLATED RAT PANCREATIC ACINI
Autore:
OTSUKI M; FUJII M; NAKAMURA T; TANI S; OKABAYASHI Y;
Indirizzi:
UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT INTERNAL MED 3,YAHATANISHI KU,1-1 ISEIGAOKA KITAKYUSHU FUKUOKA 807 JAPAN KOBE UNIV,SCH MED,DEPT INTERNAL MED 2 KOBE JAPAN
Titolo Testata:
International journal of pancreatology
fascicolo: 2, volume: 18, anno: 1995,
pagine: 135 - 143
SICI:
0169-4197(1995)18:2<135:COOSTC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEINASE-INHIBITOR; ENZYME-SECRETION; CHOLECYSTOKININ; ASSAY; ANTAGONIST; L-364,718; CERULEIN; BINDING;
Keywords:
STIMULUS-SECRETION COUPLING; CAMOSTATE; AMYLASE RELEASE; PROTEIN KINASE C; ISOLATED PANCREATIC ACINI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
M. Otsuki et al., "CHRONIC ORAL-ADMINISTRATION OF SYNTHETIC TRYPSIN-INHIBITOR CAMOSTATE REDUCES AMYLASE RELEASE FROM ISOLATED RAT PANCREATIC ACINI", International journal of pancreatology, 18(2), 1995, pp. 135-143

Abstract

In the present study, we examined stimulus-secretion coupling in pancreatic acini prepared from rats given synthetic protease inhibitor camostate at a dose of 200 mg/kg body wt by an orogastric tube once a dayfor 10 d. Camostate treatment significantly increased pancreatic weight, protein, DNA, and enzyme contents. In acini prepared from the camostate-treated rats: responsiveness to both CCK-S and carbamylcholine was greatly decreased with no shift in the dose-response curves compared to control acini prepared from saline-treated rats. There were no major changes in the affinity for both high- and low-affinity sites of CCK receptors, but there was a significant reduction in the capacity oflow-affinity site based on acinar protein. Responsiveness to secretinin the camostate-treated rat acini was also significantly reduced compared with that in the controls. However, amylase release from the camostate-treated rat acini in response to an increase in intracellular calcium levers induced by the calcium ionophores A23187 or to an increase in intracellular cyclic 3', 5'-monophosphate (cyclic AMP) levels caused by 8 bromo cyclic AMP was not significantly different from the control rat acini, suggesting that both Ca2+-dependent tyrosine kinase and nucleotide-activated kinases are not impaired. On the other hand, the responsiveness to phorbol ester TPA, which stimulates amylase secretion via a calcium-independent cascade by activating protein kinase C directly, was reduced in the camostate-treated rat acini compared withthe controls. These results suggest the possibilities that the reduced amylase secretion in the camostate-treated rats is owing to alterations in both the transmembrane signal transduction and the phosphorylation of regulatory proteins by the Ca2+-independent, protein kinase C-dependent mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:22:51