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Titolo:
STABLE INDUCIBLE EXPRESSION OF A FUNCTIONAL-RAT LIVER ORGANIC ANION TRANSPORT PROTEIN IN HELA-CELLS
Autore:
SHI XY; BAI S; FORD AC; BURK RD; JACQUEMIN E; HAGENBUCH B; MEIER PJ; WOLKOFF AW;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,MARION BESSIN LIVER RES CTR,1300 MORRIS PK AVE BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,MARION BESSIN LIVER RES CTR BRONX NY 10461 UNITE RECH F-94275 LE KREMLIN BICETR FRANCE UNIV ZURICH HOSP,DIV CLIN PHARMACOL CH-8091 ZURICH SWITZERLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 43, volume: 270, anno: 1995,
pagine: 25591 - 25595
SICI:
0021-9258(1995)270:43<25591:SIEOAF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS-LAEVIS OOCYTES; PLASMA-MEMBRANE; BINDING-PROTEIN; SERUM-ALBUMIN; BILIRUBIN; SULFOBROMOPHTHALEIN; CHLORIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
X.Y. Shi et al., "STABLE INDUCIBLE EXPRESSION OF A FUNCTIONAL-RAT LIVER ORGANIC ANION TRANSPORT PROTEIN IN HELA-CELLS", The Journal of biological chemistry, 270(43), 1995, pp. 25591-25595

Abstract

Recently we expression cloned a rat liver organic anion transport protein in Xenopus laevis oocytes (Jacquemin, E., Hagenbuch, B., Stieger,B., Wolkoff, A. W., and Meier, P. J. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 133-137). In the present study, we have stably transfected the cDNA encoding this protein into HeLa cells by using a vector containing a zinc-inducible promoter. The parent cells have virtually no baseline transport of [S-35]sulfobromophthalein, whereas the induced transfected cells express a novel 74-kDa protein and avidly transport this ligand. Transport by these cells is saturable (K-m = 3.3 mu M, V-max= 257 pmol/min/mg protein), bidirectional, and highly temperature-dependent. In the presence of albumin, uptake of [S-35]sulfobromophthalein requires the presence of extracellular Cl-, whereas in the absence of albumin, this Cl- dependence is not seen. These studies indicate that cellular uptake of sulfobromophthalein does not result hom direct interaction with the plasma membrane lipid bilayer but rather requires the presence of a specific plasma membrane transporter.

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Documento generato il 28/01/21 alle ore 07:37:26