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Titolo:
LACK OF ELEVATED DRUG EFFLUX IN ADRIAMYCIN-RESISTANT IMMUNOBLASTIC-B LYMPHOMA-CELLS WITH MDR1 OVEREXPRESSION
Autore:
CHAO CCK;
Indirizzi:
CHANG GUNG MED COLL,DEPT BIOCHEM,TUMOR BIOL LAB TAYUAN 33332 TAIWAN
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 373, anno: 1995,
pagine: 285 - 290
SICI:
0014-5793(1995)373:3<285:LOEDEI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST CANCER-CELLS; GLUTATHIONE-S-TRANSFERASE; P-GLYCOPROTEIN GENE; PROTEIN-KINASE-C; MULTIDRUG RESISTANCE; CYTO-TOXICITY; H-RAS; LINE; EXPRESSION; CISPLATIN;
Keywords:
DRUG EFFLUX; IMMUNOBLASTIC LYMPHOMA; MDR; P-GLYCOPROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
C.C.K. Chao, "LACK OF ELEVATED DRUG EFFLUX IN ADRIAMYCIN-RESISTANT IMMUNOBLASTIC-B LYMPHOMA-CELLS WITH MDR1 OVEREXPRESSION", FEBS letters, 373(3), 1995, pp. 285-290

Abstract

A multidrug-resistant (MDR) subline of the immunoblastic B lymphoma cell line was established by sequentially selecting in increasing concentrations of adriamycin, The adriamycin-resistant cell line (HOB1/ADR)demonstrated resistance to a wide spectrum of chemotherapeutic agentsincluding MDR drugs (Vinca alkaloids and anthracycline), antimicrotubule drug (colchicine), and DIVA-damaging agents (cisplatin and mitomycin C). The expression of human mdr1 gene, as analyzed by RT-PCR and Western blotting, revealed a 13-15-fold increase in resistant cells, Unexpectedly, HOB1/ADR cells demonstrated a lack of reduced accumulation and of enhanced efflux of adriamycin, More than 60% adriamycin was effluxed at the same rate in both cell lines within 10 min, In contrast, the initial rate of vincristine accumulation was reduced by 3 fold in this resistant cell line, The maximal level of vincristine accumulation was 50% lower in the resistant cells than the parental cells, The maximal efflux rate was enhanced by 5 fold in the resistant cells, Inhibition of vincristine resistance by verapamil associated with restoration of drug accumulation, suggesting that acquired resistance in these cells is due to P-glycoprotein, These studies demonstrated that immunoblastic B lymphoma cells selected for adriamycin resistance preferentially developed P-glycoprotein-mediated vincristine efflux which plays an important role in vincristine resistance, In contrast, the resistant cells did not elevate adriamycin efflux, suggesting an additional mechanism responsible for adriamycin resistance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:27:30