Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LEUKOTRIENE C-4 RECEPTORS ON GUINEA-PIG TRACHEOCYTES
Autore:
PRIE S; GUILLEMETTE G; BOULAY G; BORGEAT P; SIROIS P;
Indirizzi:
UNIV SHERBROOKE,FAC MED,DEPT PHARMACOL SHERBROOKE PQ J1H 5N4 CANADA UNIV SHERBROOKE,FAC MED,DEPT PHARMACOL SHERBROOKE PQ J1H 5N4 CANADA UNITE RECH INFLAMMAT IMMUNOL RHUMATOL QUEBEC CITY PQ CANADA
Titolo Testata:
The Journal of pharmacology and experimental therapeutics
fascicolo: 1, volume: 275, anno: 1995,
pagine: 312 - 318
SICI:
0022-3565(1995)275:1<312:LCROGT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; LUNG MEMBRANES; PHARMACOLOGICAL EVIDENCE; H-3 LEUKOTRIENE-C4; ENDOTHELIAL-CELLS; BINDING-SITES; D4 RECEPTORS; IDENTIFICATION; RAT; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
S. Prie et al., "LEUKOTRIENE C-4 RECEPTORS ON GUINEA-PIG TRACHEOCYTES", The Journal of pharmacology and experimental therapeutics, 275(1), 1995, pp. 312-318

Abstract

Leukotriene (LT) C-4 receptors have been characterized on freshly isolated guinea pig tracheal epithelial cells (tracheocytes). The [H-3]LTC(4) receptor affinity was enhanced by increasing the sodium (60-160 mM) and the magnesium (0-10 mM) concentrations. Low concentrations of calcium (0-3 mM) increased [H-3]LTC(4) binding, but high concentrations(3-10 mM) decreased it. The pH (6.5-8.0) had no effect on [H-3]LTC(4)binding to tracheocytes. Under our experimental conditions, binding equilibrium was reached after 20 min. The association and the dissociation rate constants were estimated to be 2.75 +/- 0.25 x 10(6) M(-1). min(-1) and 0.093 +/- 0.008 min(-1), respectively. The K-d(35.4 +/- 8.6nM) and the B-max values (2.4 +/- 0.6 x 10(5) receptors/cell) were determined by Scatchard analysis. LTB(4), LTD(4) and LTE(4) did not inhibit [H-3]LTC(4) binding to the receptors. However, the compound FPL 55712 inhibited the binding of [H-3]LTC(4) with an IC50 value of 9.0 +/-1.0 mu M. [H-3]LTC(4) was not metabolized during the binding assays, as confirmed by reverse-phase high-performance liquid chromatography. The lack of [H-3]LTC(4) binding to glutathione-S-transferase was demonstrated in the presence of an excess of reduced glutathione. LTC(4) produced a concentration-dependent increase of free Ca++ in tracheocytes. Our results suggest that guinea pig tracheocytes possess a specific LTC(4) receptor coupled to a Ca++ signaling pathway. This LTC(4) receptor may play a key role in the epithelium-dependent responses of airway smooth muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 14:12:47