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Titolo:
FIBROBLAST GROWTH FACTOR-II CAN MEDIATE CELL ATTACHMENT BY LINKING RECEPTORS AND HEPARAN-SULFATE PROTEOGLYCANS ON NEIGHBORING CELLS
Autore:
RICHARD C; LIUZZO JP; MOSCATELLI D;
Indirizzi:
NYU,MED CTR,DEPT CELL BIOL,550 1ST AVE NEW YORK NY 10016 NYU,MED CTR,DEPT CELL BIOL NEW YORK NY 10016 NYU,MED CTR,KAPLAN CANC CTR NEW YORK NY 10016
Titolo Testata:
The Journal of biological chemistry
fascicolo: 41, volume: 270, anno: 1995,
pagine: 24188 - 24196
SICI:
0021-9258(1995)270:41<24188:FGFCMC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; FACTOR FGF RECEPTOR; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; HEMATOPOIETIC-CELLS; PROGENITOR CELLS; LIGAND-BINDING; LEUKEMIA-CELLS; EXPRESSION; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
C. Richard et al., "FIBROBLAST GROWTH FACTOR-II CAN MEDIATE CELL ATTACHMENT BY LINKING RECEPTORS AND HEPARAN-SULFATE PROTEOGLYCANS ON NEIGHBORING CELLS", The Journal of biological chemistry, 270(41), 1995, pp. 24188-24196

Abstract

The myeloid 32D cell line, which grows in suspension and does not express FGF receptors or heparan sulfate proteoglycans, was transfected with the cDNA encoding FGF receptor-1 (32D-flg cells), When co culturedwith glutaraldehyde-fixed Chinese hamster ovary (CHO) cells, the 32D-flg cells remained in suspension in the absence of FGF-2 but attached to the CHO monolayer in the presence of 10 ng/ml FGF-2. In contrast, 32D cells transfected with the vector alone did not attach to the CHO monolayer in the presence of FGF-2, FGF-2-dependent attachment of 32D-flg cells was prevented by inclusion of 10 mu g/ml heparin in the incubation medium and was diminished when CHO mutants in glycosaminoglycan synthesis or wild-type CHO cells treated with heparinase were used, indicating that the attachment occurred through FGF-2 interactions with heparan sulfates on the CHO cells. Attachment of 32D-flg cells to wild-type CHO cells was half-maximal at 0.4 ng/ml FGF-2 and was also observed with FGF-1 but not FGF-4, 32D-flg cells also attached to living CHO cells in a FGF-S-dependent manner, but attachment was transient at 37 degrees C. Induction of new proteins was not required for FGF-2-dependent attachment, since attachment occurred when the co cultures were incubated at 4 degrees C and when the 32D-flg cells were preincubated with cycloheximide. FGF-2-dependent attachment of 32D-flg cells was also observed with Balb/C 3T3, NIH 3T3, and bovine capillary endothelialcells. We conclude that attachment is due to FGF-2 binding simultaneously to receptors on the 32D-flg cells and heparan sulfates on the CHOmonolayers; thus, the FGF-P acts as a bridge between receptor-expressing cells and heparan sulfate-bearing cells. In addition, induction ofDNA synthesis in 32D-flg cells in response to FGF-2 was potentiated by the CHO-associated heparan sulfates to the same extent as by solubleheparin, indicating that this interaction has functional significance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:11:33