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Titolo:
PRODUCTION, PURIFICATION AND CHARACTERIZATION OF GLUCOSE-OXIDASE FROMPENICILLIUM VARIABILE P16
Autore:
GARZILLO AMV; DIPAOLO S; FENICE M; PETRUCCIOLI M; BUONOCORE V; FEDERICI F;
Indirizzi:
UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,VIA SC DE LELLIS I-01100VITERBO ITALY UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM I-01100 VITERBO ITALY UNIV BASILICATA,DIPARTIMENTO BIOL DIFESA & BIOTECNOL AGROFORESTAL I-85100 POTENZA ITALY
Titolo Testata:
Biotechnology and applied biochemistry
, volume: 22, anno: 1995,
parte:, 2
pagine: 169 - 178
SICI:
0885-4513(1995)22:<169:PPACOG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
A.M.V. Garzillo et al., "PRODUCTION, PURIFICATION AND CHARACTERIZATION OF GLUCOSE-OXIDASE FROMPENICILLIUM VARIABILE P16", Biotechnology and applied biochemistry, 22, 1995, pp. 169-178

Abstract

Glucose oxidase was produced by Penicillium variabile P16 grown in a benchtop bioreactor and purified 30-fold to homogeneity by ion-exchange and gel-filtration chromatography. The M(r) of the native enzyme was126 000 and that of the subunit 62 000, which indicated that the native enzyme is a dimer. The enzyme was optimally active at pH 6.0 and 55degrees C and highly specific for beta-D-glucose (K-m 6.0 mM). Two isoenzymes, with pI values of 4.8-4.9, were detected on analytical isoelectric-focusing gels. The glucose oxidase was severely inhibited by Cu2+ and Ag+ ions and, to a lower extent, by NaF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:41:29