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Titolo:
THE RESPONSE OF A CYTOKININ RESISTANT MUTANT IS HIGHLY SPECIFIC AND PERMITS A NEW CYTOKININ BIOASSAY
Autore:
NOGUE F; JULLIEN M; MORNET R; LALOUE M;
Indirizzi:
INRA,BIOL CELLULAIRE LAB F-78000 VERSAILLES FRANCE FAC SCI ANGERS,CHIM FONDAMENTALE & APPL LAB F-49045 ANGERS FRANCE
Titolo Testata:
Plant growth regulation
fascicolo: 2, volume: 17, anno: 1995,
pagine: 87 - 94
SICI:
0167-6903(1995)17:2<87:TROACR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICOTIANA-PLUMBAGINIFOLIA; REGULATORS; COTYLEDONS; AUXIN;
Keywords:
CYTOKININ; CYTOKININ-BIOASSAY; NICOTIANA PLUMBAGINIFOLIA; RESISTANT MUTANT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
F. Nogue et al., "THE RESPONSE OF A CYTOKININ RESISTANT MUTANT IS HIGHLY SPECIFIC AND PERMITS A NEW CYTOKININ BIOASSAY", Plant growth regulation, 17(2), 1995, pp. 87-94

Abstract

We have previously isolated six independent cytokinin-resistant mutants of Nicotiana plumbaginifolia which define three complementation groups, zea1, zea2 and zea3. We report here the characterization of the phenotypic response to cytokinin treatment of the mutant 1-64, belonging to the zeal group, and the result of the study of the specificity ofthis response. The phenotype of this mutant grown in the presence of cytokinin concentrations higher than 0.1 mu M is characterized by a hypertrophy of the cotyledons and hypocotyl which results in an increaseof plantlet fresh weight. This hypertrophy is correlated to cytokininconcentration in a range between 0.01 to 10 mu M. The specificity of this response has been verified by using adenine and urea type cytokinins, as well as enantiomers of methylzeatin and methylbenzyladenine which differ widely in their cytokinin activities. We show that the highspecificity of the hypertrophic response to cytokinins can be used asa convenient bioassay to screen the cytokinin activity of adenine or urea type molecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:33:43