Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
GENE-REGULATORY MODULES IN ESCHERICHIA-COLI - NUCLEOPROTEIN COMPLEXESFORMED BY CAMP-CRP AND CYTR AT THE NUPG PROMOTER
Autore:
PEDERSEN H; DALL J; DANDANELL G; VALENTINHANSEN P;
Indirizzi:
ODENSE UNIV,DEPT MOLEC BIOL,CAMPUSVEJ 55 DK-5230 ODENSE M DENMARK ODENSE UNIV,DEPT MOLEC BIOL DK-5230 ODENSE M DENMARK UNIV COPENHAGEN,INST BIOL CHEM DK-1307 COPENHAGEN K DENMARK
Titolo Testata:
Molecular microbiology
fascicolo: 5, volume: 17, anno: 1995,
pagine: 843 - 853
SICI:
0950-382X(1995)17:5<843:GMIE-N>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMP RECEPTOR PROTEIN; DEOP2 PROMOTER; CYCLIC-AMP; ACTIVATOR PROTEIN; REPRESSOR PROTEIN; BINDING-SITES; DNA; TRANSCRIPTION; SPECIFICITY; DISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
H. Pedersen et al., "GENE-REGULATORY MODULES IN ESCHERICHIA-COLI - NUCLEOPROTEIN COMPLEXESFORMED BY CAMP-CRP AND CYTR AT THE NUPG PROMOTER", Molecular microbiology, 17(5), 1995, pp. 843-853

Abstract

Repression by CytR depends on the formation of nucleoprotein complexes in which the CytR repressor and the cAMP-CRP activator complex bind cooperatively to the DNA. Transcription initiation from CytR-regulatedpromoters requires cAMP-CRP; therefore, the cAMP-CRP complex functions both as an activator and as a co-repressor in these promoters, Another interesting aspect of the CytR regulon is that each promoter appears to have individual features. Therefore, structural and functional rules governing the formation of repression and activation complexes in one promoter may not be valid for other promoters of the CytR regulon. Here we show that the Escherichia coli nupG gene contains one CytR- and four CRP-binding sites in the control region. Notably, the architecture of the CytR binding site is different from previously described targets. In addition, the CytR repressor triggers a DNA repositioning of a cAMP-CRP complex in the -35 region upon binding to its operator. Thus, formation of the repression and activation complexes at the nupG promoter involves different subsets of CRP-binding sites. These findings show that the bacterium uses positive and negative regulatory modules to differentially control the expression of CytR- and cAMP-CRP-regulated genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:52:44