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Titolo:
IMMUNOLOCALIZATION OF TRIADIN, DHP RECEPTORS, AND RYANODINE RECEPTORSIN ADULT AND DEVELOPING SKELETAL-MUSCLE OF RATS
Autore:
CARL SL; FELIX K; CASWELL AH; BRANDT NR; BRUNSCHWIG JP; MEISSNER G; FERGUSON DG;
Indirizzi:
CASE WESTERN RESERVE UNIV,DEPT ANAT,10900 EUCLID AVE CLEVELAND OH 44106 UNIV CINCINNATI,COLL MED,DEPT MOLEC & CELLULAR PHYSIOL CINCINNATI OH 45267 UNIV MIAMI,DEPT PHARMACOL MIAMI FL 33152 UNIV N CAROLINA,DEPT BIOCHEM CHAPEL HILL NC 27599
Titolo Testata:
Muscle & nerve
fascicolo: 11, volume: 18, anno: 1995,
pagine: 1232 - 1243
SICI:
0148-639X(1995)18:11<1232:IOTDRA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETICULUM GLYCOPROTEIN TRIADIN; JUNCTIONAL FOOT PROTEIN; CA-2+ RELEASE CHANNEL; SARCOPLASMIC-RETICULUM; DIHYDROPYRIDINE RECEPTOR; 1,4-DIHYDROPYRIDINE RECEPTOR; TRANSVERSE TUBULES; COMPLEMENTARY-DNA; LOCALIZATION; EXPRESSION;
Keywords:
EXCITATION CONTRACTION COUPLING; SKELETAL MUSCLE; TRIAD JUNCTIONS; TRIADIN; DHP RECEPTOR; RYANODINE RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S.L. Carl et al., "IMMUNOLOCALIZATION OF TRIADIN, DHP RECEPTORS, AND RYANODINE RECEPTORSIN ADULT AND DEVELOPING SKELETAL-MUSCLE OF RATS", Muscle & nerve, 18(11), 1995, pp. 1232-1243

Abstract

The dihydropyridine receptors (DHPR) and the ryanodine receptors (RyR) are well-characterized proteins of the triad junctions of skeletal muscle fibers. Recently, a newly discovered 95-kDa protein, triadin, has been purified from rabbit skeletal muscle heavy sarcoplasmic reticulum (SR) vesicles, WE have used indirect immunogold EM to localize triadin to the junctional face of the SR in isolated triads. In addition, we have used indirect immunofluorescence to localize triadin in relation to the DHPR and the RyR in adult and developing rat skeletal muscle. In double immunolabeling experiments of longitudinally oriented adult rat skeletal muscle tissue, triadin-specific and RyR-specific antibodies resulted in a characteristic striated staining pattern. The staining arising from these antibodies completely overlapped when examined by computer analysis of digitized laser scanning confocal microscopy images. A similar result was obtained in double staining experiments using antibodies raised against the DHPR and the RyR suggesting that allthree proteins are located in the triads in situ. The developmental expression of the three triad proteins was examined using double labeling of skeletal muscle tissue from several fetal and early postnatal ages. The staining patterns of triadin, RyR, and DHPR antibodies were overlapping throughout development, suggesting that from their earliest appearance the three proteins are components of the triads. (C) 1995 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:29:07