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Titolo:
SUBUNIT COMPOSITION OF G(O) PROTEINS FUNCTIONALLY COUPLING GALANIN RECEPTORS TO VOLTAGE-GATED CALCIUM CHANNELS
Autore:
KALKBRENNER F; DEGTIAR VE; SCHENKER M; BRENDEL S; ZOBEL A; HESCHLER J; WITTIG B; SCHULTZ G;
Indirizzi:
FREE UNIV BERLIN,INST PHARMAKOL,THIELALLEE 69-73 D-14195 BERLIN GERMANY FREE UNIV BERLIN,INST MOLEK BIOL & BIOCHEM D-14195 BERLIN GERMANY
Titolo Testata:
EMBO journal
fascicolo: 19, volume: 14, anno: 1995,
pagine: 4728 - 4737
SICI:
0261-4189(1995)14:19<4728:SCOGPF>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSULIN-SECRETING CELLS; BETA-GAMMA-DIMERS; MOLECULAR-CLONING; HORMONE-RELEASE; ALPHA-SUBUNITS; CA2+ CURRENTS; RINM5F CELLS; K+ CHANNELS; CDNA; INHIBITION;
Keywords:
ANTISENSE; CA2+ CHANNEL REGULATION; GALANIN EFFECTS; G PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
F. Kalkbrenner et al., "SUBUNIT COMPOSITION OF G(O) PROTEINS FUNCTIONALLY COUPLING GALANIN RECEPTORS TO VOLTAGE-GATED CALCIUM CHANNELS", EMBO journal, 14(19), 1995, pp. 4728-4737

Abstract

The neuropeptide galanin is widely expressed in the central nervous system and other tissues and induces different cellular reactions, e.g.hormone release from pituitary and inhibition of insulin release frompancreatic B cells. By microinjection of antisense oligonucleotides we studied the question as to which G proteins mediate the galanin-induced inhibition of voltage-gated Ca2+ channels in the rat pancreatic B-cell line RINm5F and in the rat pituitary cell line GH(3). Injection of antisense oligonucleotides directed against alpha(o1), beta(2), beta(3), gamma(2), and gamma(4) G protein subunits reduced the inhibition of Ca2+ channel current which was induced by galanin, whereas no change was seen after injection of cells with antisense oligonucleotides directed against alpha(i), alpha(q), alpha(11), alpha(14), alpha(15), beta(1), beta(4), gamma(1), gamma(3), gamma(5), or gamma(7) G protein subunits or with sense control oligonucleotides. In view of these data and of previous results, we conclude that the galanin receptors in GH(3) and in RINm5F cells couple mainly to the G(o) protein consisting of alpha(o1)beta(2) gamma(2), to inhibit Ca2+ channels and use alpha(o1)beta(3) gamma(4) less efficiently. The latter G protein composition waspreviously shown to be used by muscarinic M(4) receptors to inhibit Ca2+ channels.

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Documento generato il 25/11/20 alle ore 09:36:49