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Titolo:
STRUCTURE AND EXPRESSION OF AN OVINE ENDOMETRIAL OXYTOCIN RECEPTOR CDNA
Autore:
RILEY PR; FLINT APF; ABAYASEKARA DRE; STEWART HJ;
Indirizzi:
ZOOL SOC LONDON,INST ZOOL,REGENTS PK LONDON NW1 4RY ENGLAND UNIV NOTTINGHAM,DEPT PHYSIOL & ENVIRONM SCI LOUGHBOROUGH LE12 5RD LEICS ENGLAND ROYAL FREE HOSP,SCH MED,DEPT PHYSIOL LONDON NW3 2PF ENGLAND BABRAHAM INST,DEPT CELLULAR PHYSIOL CAMBRIDGE CB2 4AT ENGLAND
Titolo Testata:
Journal of molecular endocrinology
fascicolo: 2, volume: 15, anno: 1995,
pagine: 195 - 202
SICI:
0952-5041(1995)15:2<195:SAEOAO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; PROTEIN; VASOPRESSIN; PREGNANCY; BINDING; HORMONE; SHEEP; PROSTAGLANDIN; SEQUENCES; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
P.R. Riley et al., "STRUCTURE AND EXPRESSION OF AN OVINE ENDOMETRIAL OXYTOCIN RECEPTOR CDNA", Journal of molecular endocrinology, 15(2), 1995, pp. 195-202

Abstract

A sheep endometrial oxytocin receptor (OTR) cDNA (1.5 kb) was isolated from a lambda-ZAP library using a reverse transcription-PCR product probe generated from oestrous endometrial mRNA. The sheep OTR cDNA shared an overall similarity of 82% with human OTR cDNA, 85% with pig OTRcDNA and 76% with rat OTR cDNA. The encoded receptor was a 398 amino acid polypeptide 94% similar to human OTR, 94% similar to pig OTR and 93% similar to rat OTR. The sheep OTR contained two additional amino acids compared with human OTR which were located in the highly GC-rich third intracytoplasmic loop. This region is thought to be associated with G protein coupling and signal transduction. Expression of the cDNAin Cos-7 cells and measurement of oxytocin-induced phosphoinositide turnover confirmed that it coded for a functional product. The affinityof the expressed receptor was comparable with that observed for the in vivo receptor.

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Documento generato il 30/10/20 alle ore 09:46:43