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Titolo:
REPETITIVE SPERM-INDUCED CA2-CYCLE DEPENDENT( TRANSIENTS IN MOUSE OOCYTES ARE CELL)
Autore:
JONES KT; CARROLL J; MERRIMAN JA; WHITTINGHAM DG; KONO T;
Indirizzi:
ST GEORGE HOSP,SCH MED,MRC,EXPTL EMBRYOL & TERATOL UNIT,CRANMER TERRACE LONDON SW17 0RE ENGLAND
Titolo Testata:
Development
fascicolo: 10, volume: 121, anno: 1995,
pagine: 3259 - 3266
SICI:
0950-1991(1995)121:10<3259:RSCDTI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAPHASE-II ARREST; HAMSTER EGGS; CALCIUM OSCILLATIONS; PARTHENOGENETIC ACTIVATION; MEIOTIC MATURATION; PROTEIN-SYNTHESIS; CA-2+; INVITRO; FERTILIZATION; PROGRESSION;
Keywords:
CYCLOHEXIMIDE; MITOSIS; MEIOSIS; FERTILIZATION; MOUSE OOCYTE; CA2+ TRANSIENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
K.T. Jones et al., "REPETITIVE SPERM-INDUCED CA2-CYCLE DEPENDENT( TRANSIENTS IN MOUSE OOCYTES ARE CELL)", Development, 121(10), 1995, pp. 3259-3266

Abstract

Mature mouse oocytes are arrested at metaphase of the second meiotic division, Completion of meiosis and a block to polyspermy is caused bya series of repetitive Ca2+ transients triggered by the sperm at fertilization. These Ca2+ transients have been widely reported to last fora number of hours but when, or why, they cease is not known. Here we show that Ca2+ transients cease during entry into interphase, at the time when pronuclei are forming, In fertilized oocytes arrested at metaphase using colcemid, Ca2+ transients continued for as long as measurements were made, up to 18 hours after fertilization, Therefore sperm is able to induce Ca2+ transients during metaphase but not during interphase, In addition metaphase II oocytes, but not pronuclear stage 1-cell embryos showed highly repetitive Ca2+ oscillations in response to microinjection of inositol trisphosphate, This was explored further by treating in vitro maturing oocytes at metaphase I for 4-5 hours with cycloheximide, which induced nuclear progression to interphase (nucleusformation) and subsequent re-entry to metaphase (nuclear envelope breakdown), Fertilization of cycloheximide-treated oocytes revealed that continuous Ca2+ oscillations in response to sperm were observed after nuclear envelope breakdown but not during interphase, However interphase oocytes were able to generate Ca2+ transients in response to thimerosal, This data suggests that the ability of the sperm to trigger repetitive Ca2+ transients in oocytes is modulated in a cell cycle-dependent manner.

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Documento generato il 28/11/20 alle ore 21:40:27