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Titolo:
MEMBRANE EXPRESSION OF HLA-CW4 FREE CHAINS IN ACTIVATED T-CELLS OF TRANSGENIC MICE
Autore:
AIUTI A; FORTE P; SIMEONI L; LINO M; POZZI L; FATTOROSSI A; GIACOMINI P; GINELLI E; BERETTA A; SICCARDI A; FANTONI A;
Indirizzi:
UNIV ROMA LA SAPIENZA,DIPARTIMENTO BIOPATOL UMANA,SEZ BIOL CELLULARE,VIALE REGINA ELENA 324 I-00161 ROME ITALY UNIV ROMA LA SAPIENZA,DIPARTIMENTO BIOPATOL UMANA,SEZ BIOL CELLULARE I-00161 ROME ITALY DASRS,IMMUNOL LAB I-00040 POMEZIA ITALY IST REGINA ELENA,CRS,IMMUNOL LAB I-00158 ROME ITALY UNIV MILAN,DIP BIOL & GENET SCI MED I-20133 MILAN ITALY DIBIT HSR I-20132 MILAN ITALY
Titolo Testata:
Immunogenetics
fascicolo: 5, volume: 42, anno: 1995,
pagine: 368 - 375
SICI:
0093-7711(1995)42:5<368:MEOHFC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HLA-C LOCUS; CLASS-I MOLECULES; HEAVY-CHAINS; SURFACE EXPRESSION; BETA-2-MICROGLOBULIN; ANTIGENS; CLONE; DEFINITION; PRODUCTS; GENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
A. Aiuti et al., "MEMBRANE EXPRESSION OF HLA-CW4 FREE CHAINS IN ACTIVATED T-CELLS OF TRANSGENIC MICE", Immunogenetics, 42(5), 1995, pp. 368-375

Abstract

Transgenic mice were produced in which human HLA-Cw4 is stably integrated, behaves as a single Mendelian trait, and, being under the transcriptional control of human CD2, is selectively and efficiently expressed in T lymphocytes. These mice were used as a model system to determine whether HLA-type C molecules can be exposed on the surface of activated lymphocytes as free heavy chains, non-associated with beta 2-microglobulin (beta(2)m) In our transgenic mice we could identify HLA-Cw4 molecules either as free chains or as beta(2)m-associated molecules bythe use of monoclonal antibodies specific for either conformation of HLA class I and nonreactive to mouse H2 molecules. Resting mouse lymphocytes were shown by western transfer analysis to contain sizeable amounts of HLA-Cw4 free chains, but they exposed on their surface HLA-Cw4only in association with beta(2)m, as indicated by flow cytometric measurements, Conversely, where the content of total HLA-Cw4 was increased, lectin-activated mouse lymphocytes exposed on their outer cell membrane HLA-Cw4 molecules in both conformations, namely, also as free heavy chains. Isoelectrofocusing analysis confirmed the presence of bothHLA-Cw4 molecular conformations in activated T cells and indicated that HLA-Cw4 heavy chains can bind to mouse beta(2)m with the same low affinity displayed for human beta(2)m. The results of our experiments led us to conclude that (1) association with beta(2)m is not necessary for the exposure of HLA-C on the surface of activated T lymphocytes and (2) cell activation affects the balance between the two conformational forms of HLA-C.

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Documento generato il 09/07/20 alle ore 16:08:44