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Titolo:
IMMUNOHISTOCHEMICAL LOCALIZATION OF SPERMADHESIN AWN IN THE PORCINE MALE GENITAL-TRACT
Autore:
SINOWATZ F; AMSELGRUBER W; TOPFERPETERSEN E; CALVETE JJ; SANZ L; PLENDL J;
Indirizzi:
UNIV MUNICH,LEHRSTUHL TIERANAT 2,VET STR 13 D-80539 MUNICH GERMANY HANNOVER SCH VET MED,INST REPROD MED W-3000 HANNOVER GERMANY
Titolo Testata:
Cell and tissue research
fascicolo: 1, volume: 282, anno: 1995,
pagine: 175 - 179
SICI:
0302-766X(1995)282:1<175:ILOSAI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PELLUCIDA-BINDING PROTEIN; ZONA-PELLUCIDA; BIOCHEMICAL-CHARACTERIZATION; BOAR SPERMATOZOA; SPERM; FERTILIZATION; ISOFORMS; EGG;
Keywords:
IMMUNOHISTOCHEMISTRY; ZONA PELLUCIDA BINDING PROTEIN; BOAR SPERMADHESIN; PIG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
F. Sinowatz et al., "IMMUNOHISTOCHEMICAL LOCALIZATION OF SPERMADHESIN AWN IN THE PORCINE MALE GENITAL-TRACT", Cell and tissue research, 282(1), 1995, pp. 175-179

Abstract

Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attachedto the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of spermatozoa was also uncertain. By using monospecific polyclonal antibodies against AWN, the immunohistochemical distribution pattern of AWN epitopes has been investigated in tissue sections of the porcine male genital tract. Our study has revealed that AWN is synthesized in the rete testis and in the epithelium ofthe seminal vesicles. The latter are also the major contributors of seminal plasma AWN. In addition, immunoblotting analysis has shown thatAWN is present on epididymal spermatozoa. Our results indicate that the cellular origin of sperm-adhesins is species-specific. The attachment of AWN to epididymal spermatozoa is probably important in developing the capacity for fertilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 16:04:09