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Titolo:
THE BINDING MOTIFS FOR AC TRANSPOSASE ARE ABSOLUTELY REQUIRED FOR EXCISION OF DS1 IN MAIZE
Autore:
BRAVOANGEL AM; BECKER HA; KUNZE R; HOHN B; SHEN WH;
Indirizzi:
FRIEDRICH MIESCHER INST,POB 2543 CH-4002 BASEL SWITZERLAND UNIV COLOGNE,INST GENET D-50931 COLOGNE GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 5, volume: 248, anno: 1995,
pagine: 527 - 534
SICI:
0026-8925(1995)248:5<527:TBMFAT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTROLLING ELEMENT ACTIVATOR; ZEA-MAYS-L; STREAK VIRUS; PUTATIVE TRANSPOSASE; NUCLEOTIDE-SEQUENCE; MOLECULAR ANALYSIS; PLANTS; TOBACCO; DNA; AGROINFECTION;
Keywords:
DS1 EXCISION; MAIZE STREAK VIRUS; ZEA MAYS PLANTS; AGROINFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Bravoangel et al., "THE BINDING MOTIFS FOR AC TRANSPOSASE ARE ABSOLUTELY REQUIRED FOR EXCISION OF DS1 IN MAIZE", MGG. Molecular & general genetics, 248(5), 1995, pp. 527-534

Abstract

A reverse genetic system for studying excision of the transposable element Ds1 in maize plants has been established previously. In this system, the Ds1 element, as part of the genome of maize streak virus (MSV), is introduced into maize plants via agroinfection. In the presence of the Ac element, excision of Ds1 from the MSV genome results in the appearance of viral symptoms on the maize plants. Here, we used this system to study DNA sequences required in cis for excision of Ds1. The Ds1 element contains the Ac transposase binding motif AAACGG in only one of its subterminal regions (defined here as the 5' subterminal region). We showed that mutation of these motifs abolished completely the excision capacity of Ds1. This is the first direct demonstration that the transposase binding motifs are essential for excision. Mutagenesiswith oligonucleotide insertions in the other (3') subterminal region resulted in elements with either a reduced or an increased excision efficiency, indicating that this subterminal region also has an important function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:55:31