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Titolo:
BLOOD AND PLASMA GLUTATHIONE MEASURED IN HEALTHY-SUBJECTS BY HPLC - RELATION TO SEX, AGING, BIOLOGICAL VARIABLES, AND LIFE HABITS
Autore:
MICHELET F; GUEGUEN R; LEROY P; WELLMAN M; NICOLAS A; SIEST G;
Indirizzi:
CTR MED PREVENT,2 AVE DOYEN PARISOT F-54500 VANDOEUVRE NANCY FRANCE CTR MED PREVENT F-54500 VANDOEUVRE NANCY FRANCE FAC PHARM NANCY,CHIM ANALYT LAB F-54000 NANCY FRANCE MED CTR,CNRS,URA 597 F-54000 NANCY FRANCE
Titolo Testata:
Clinical chemistry
fascicolo: 10, volume: 41, anno: 1995,
pagine: 1509 - 1517
SICI:
0009-9147(1995)41:10<1509:BAPGMI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSOMAL LIPID-PEROXIDATION; INTRACELLULAR GLUTATHIONE; HUMAN-ERYTHROCYTES; VITAMIN-E; DEFICIENCY; DISULFIDE; DISEASE; LIVER; CELLS; CHEMOTHERAPY;
Keywords:
BIOLOGICAL VARIATION; GAMMA-GLUTAMYL-TRANSFERASE; ANTIOXIDANTS; CHOLESTEROL; FREE RADICALS; SEX- AND AGE-RELATED EFFECTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
F. Michelet et al., "BLOOD AND PLASMA GLUTATHIONE MEASURED IN HEALTHY-SUBJECTS BY HPLC - RELATION TO SEX, AGING, BIOLOGICAL VARIABLES, AND LIFE HABITS", Clinical chemistry, 41(10), 1995, pp. 1509-1517

Abstract

We report an HPLC method for measuring the concentrations of reduced (GSH) and total (GSHt) free glutathione in human plasma and whole blood. The chromatographic step was coupled with a postcolumn derivatization reaction and fluorometric detection. The linear range was 0.81-13.02 mu mol/L, and the detection limit was 0.13 mu mol/L. In healthy adults (ages 18-73 years), mean concentrations were 941 +/- 155 mu mol/L for GSHt and 849 +/- 63 mu mol/L for GSH in blood (107 men, 94 women), and 3.39 +/- 1.04 mu mol/L for GSH in plasma (66 men, 58 women). BloodGSHt but not GSH was significantly lower in children (32 boys, 32 girls: 872 +/- 157 mu mol/L) than in adults. Blood GSHt and GSH appeared to be correlated positively with the number of cigarettes smoked per day and the regular practice of physical exercise, and negatively with alcohol abstinence. We observed positive correlations between blood GSHt and cholesterol and calcium concentrations, and between blood GSH and cholesterol concentration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 06:41:32