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Titolo:
DEMONSTRATION OF VIRUS-SPECIFIC CD8(-CELLS IN MEASLES-SEROPOSITIVE INDIVIDUALS BY IN-VITRO PEPTIDE STIMULATION() MEMORY T)
Autore:
NANAN R; CARSTENS C; KRETH HW;
Indirizzi:
UNIV WURZBURG,KINDERKLIN,JOSEF SCHNEIDER STR 2 D-97080 WURZBURG GERMANY
Titolo Testata:
Clinical and experimental immunology
fascicolo: 1, volume: 102, anno: 1995,
pagine: 40 - 45
SICI:
0009-9104(1995)102:1<40:DOVCIM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIC LYMPHOCYTES-T; CEREBROSPINAL-FLUID; MULTIPLE-SCLEROSIS; PRECISE PREDICTION; ANCHOR RESIDUES; MATRIX PROTEIN; IDENTIFICATION; EPITOPE; ANTIGEN; BINDING;
Keywords:
MEASLES VIRUS; CTL EPITOPE PREDICTION; HLA-A2.1; MEMORY CD8(+) CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R. Nanan et al., "DEMONSTRATION OF VIRUS-SPECIFIC CD8(-CELLS IN MEASLES-SEROPOSITIVE INDIVIDUALS BY IN-VITRO PEPTIDE STIMULATION() MEMORY T)", Clinical and experimental immunology, 102(1), 1995, pp. 40-45

Abstract

CD8(+) cytotoxic T lymphocytes (CTL) in measles virus infection have been difficult to investigate due to the strong immunosuppressive effects exhibited by infectious measles virus in vitro. In order to circumvent immunosuppression we used predicted peptide epitopes to induce measles virus-specific CTL. This was done by screening the structural proteins of measles virus for HLA-A2.1 peptide-binding motifs with valine in position 2 and leucine in position 9. Synthetic peptides np210-218, np226-234, and np340-348 from the nucleoprotein, peptide hp29-37 from the haemagglutinin protein, and peptide pp 519-527 from the polymerase protein were synthesized and used to expand measles virus-specificCD8(+) CTL in vitro. Induction of CTL with synthetic peptides was restricted to HLA-A2-positive peripheral blood mononuclear cells (PBMC) from measles-seropositive individuals. We conclude that this method is a useful tool to demonstrate memory CD8(+) CTL in measles-seropositiveadults and to evaluate the role of structural proteins in CTL responses against measles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 15:10:21