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Titolo:
TRANSFER OF RHEUMATOID-ARTHRITIS INTO SEVERE COMBINED IMMUNODEFICIENTMICE - THE PATHOGENETIC IMPLICATIONS OF T-CELL POPULATIONS OLIGOCLONALLY EXPANDING IN THE RHEUMATOID JOINTS
Autore:
MIMA T; SAEKI Y; OHSHIMA S; NISHIMOTO N; MATSUSHITA M; SHIMIZU M; KOBAYASHI Y; NOMURA T; KISHIMOTO T;
Indirizzi:
OSAKA UNIV,SCH MED,DEPT MED 3,2-2 YAMADAOKA SUITA OSAKA 565 JAPAN OSAKA UNIV,SCH MED,DEPT MED 3 SUITA OSAKA 565 JAPAN OSAKA MINAMI NATL HOSP OSAKA 586 JAPAN OSAKA WELF ANNUITY HOSP OSAKA 553 JAPAN CENT INST EXPTL ANIM KANAGAWA 216 JAPAN
Titolo Testata:
The Journal of clinical investigation
fascicolo: 4, volume: 96, anno: 1995,
pagine: 1746 - 1758
SICI:
0021-9738(1995)96:4<1746:TORISC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN-INDUCED ARTHRITIS; SYNOVIAL-FLUID; MONOCLONAL-ANTIBODY; RECEPTOR REPERTOIRE; CLASS-II; SUSCEPTIBILITY; HETEROGENEITY; SUPERANTIGEN; LYMPHOCYTES; EXPRESSION;
Keywords:
SYNOVIAL INFILTRATING T CELL; SYNOVIAL HYPERPLASIA; REVERSE TRANSCRIPTASE PCR; T CELL RECEPTOR V-BETA REPERTOIRE; SUPERANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
T. Mima et al., "TRANSFER OF RHEUMATOID-ARTHRITIS INTO SEVERE COMBINED IMMUNODEFICIENTMICE - THE PATHOGENETIC IMPLICATIONS OF T-CELL POPULATIONS OLIGOCLONALLY EXPANDING IN THE RHEUMATOID JOINTS", The Journal of clinical investigation, 96(4), 1995, pp. 1746-1758

Abstract

To investigate the pathogenicity of T cells infiltrating in the rheumatoid joints, mononuclear cells (MNC), predominantly T cells, isolatedfrom either synovial fluid or synovial tissues of the patients with RA were transferred into severe combined immunodeficient (SCID) mice byintraarticular injections. According to our observations in this experimental system, patients with RA could be classified into at least two groups. In one group of patients, the infiltrating MNC induced synovial hyperplasia in the recipient SCID mice (the positive group), Whereas, in the other group no synovial hyperplasia was observed (the negative group), The induction of synovial hyperplasia observed in the positive group was prevented by an anti-human CD3 antibody (OKT3), indicating T cell mediation, Analysis of T cell receptor (TCR) V beta usage by reverse transcriptase polymerase chain reaction in the infiltrating MNC transferred into SCID mice revealed a marked skew towards the preferential use of certain V beta genes, which was not seen in the peripheral blood MNC, in only the positive group. The patterns of TCR/V betaskew mere not uniform among the patients, The analysis of the PCR-amplified genes of such skewed TCR/V beta by single strand conformationalpolymorphism showed distinct bands, indicating that the T cell populations expanding in rheumatoid joints of the positive group were oligoclonal, Furthermore, the enrichment of the T cell populations expressing such skewed TCR/V beta by in vitro stimulation of peripheral blood MNC of the patients with the relevant superantigen enabled the induction of synovial hyperplasia in the SCID mice, These results suggest thatthe pathogenic T cells could be activated locally in rheumatoid joints by certain antigens in some, but not in all patients with RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:38:21