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Titolo:
RECOMBINANT DUCK INTERFERON - A NEW REAGENT FOR STUDYING THE MODE OF INTERFERON ACTION AGAINST HEPATITIS-B VIRUS
Autore:
SCHULTZ U; KOCK J; SCHLICHT HJ; STAEHELI P;
Indirizzi:
UNIV FREIBURG,INST MED MICROBIOL & HYG,DEPT VIROL,HUGSTETTER STR 55 D-79008 FREIBURG GERMANY UNIV FREIBURG,INST MED MICROBIOL & HYG,DEPT VIROL D-79008 FREIBURG GERMANY UNIV ULM,DEPT VIROL D-89081 ULM GERMANY
Titolo Testata:
Virology
fascicolo: 2, volume: 212, anno: 1995,
pagine: 641 - 649
SICI:
0042-6822(1995)212:2<641:RDI-AN>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENES; POLYMERASE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
U. Schultz et al., "RECOMBINANT DUCK INTERFERON - A NEW REAGENT FOR STUDYING THE MODE OF INTERFERON ACTION AGAINST HEPATITIS-B VIRUS", Virology, 212(2), 1995, pp. 641-649

Abstract

Although interferon is widely used to treat chronic hepatitis B virusinfections, its mode of action against hepadnaviruses is largely unknown. This deficit is due mainly to the lack of suitable model systems. The duck system could not be used because purified duck interferon was not available in sufficient quantities. We have now cloned a DNA fragment that contains an intronless gene for duck interferon. The primary translation product consists of 191 amino acids, the N-terminal 30 residues of which constitute a signal peptide. Mature duck interferon is 50% identical to the recently cloned chicken interferon. Sequence homology to mammalian interferons is marginal, but conservation of four cysteine residues and inducibility by virus indicate a distant relationship between duck interferon and mammalian type I interferons. Purified recombinant duck interferon from Escherichia coli is biologically active: it activates the interferon-inducible Mx gene, prevents cell destruction by cytolytic RNA viruses, and has a strong inhibitory effecton duck hepatitis B virus in cultured primary duck hepatocytes. This new reagent should help to define the interferon-sensitive step of thehepadnavirus life cycle. Furthermore, the duck system can now be usedfor systematic studies of the in vivo effectiveness of interferon in chronic hepatitis B virus infections. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 10:23:03