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Titolo:
GROWTH ADVANTAGE AND VASCULARIZATION INDUCED BY BASIC FIBROBLAST GROWTH-FACTOR OVEREXPRESSION IN ENDOMETRIAL HEC-1-B CELLS - AN EXPORT-DEPENDENT MECHANISM OF ACTION
Autore:
COLTRINI D; GUALANDRIS A; NELLI EE; PAROLINI S; MOLINARITOSATTI MP; QUARTO N; ZICHE M; GIAVAZZI R; PRESTA M;
Indirizzi:
UNIV BRESCIA,SCH MED,DEPT BIOMED SCI & BIOTECHNOL,GEN PATHOL & IMMUNOL UNIT,VIA VALSABBINA 19 I-25123 BRESCIA ITALY UNIV BRESCIA,SCH MED,DEPT BIOMED SCI & BIOTECHNOL,GEN PATHOL & IMMUNOL UNIT I-25123 BRESCIA ITALY UNIV BRESCIA,SCH MED,DEPT BIOMED SCI & BIOTECHNOL,HISTOL UNIT I-25123BRESCIA ITALY NYU,MED CTR,DEPT CELL BIOL NEW YORK NY 10016 NYU,MED CTR,KAPLAN CANC CTR NEW YORK NY 10016 DEPT PHARMACOL I-50134 FLORENCE ITALY MARIO NEGRI INST PHARMACOL RES I-24125 BERGAMO ITALY
Titolo Testata:
Cancer research
fascicolo: 20, volume: 55, anno: 1995,
pagine: 4729 - 4738
SICI:
0008-5472(1995)55:20<4729:GAAVIB>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; CAPILLARY ENDOTHELIAL-CELLS; IMMUNOHISTOCHEMICAL LOCALIZATION; EXTRACELLULAR-MATRIX; MESSENGER-RNA; TUMOR-GROWTH; FACTOR BFGF; RELEASE; CANCER; MODULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
73
Recensione:
Indirizzi per estratti:
Citazione:
D. Coltrini et al., "GROWTH ADVANTAGE AND VASCULARIZATION INDUCED BY BASIC FIBROBLAST GROWTH-FACTOR OVEREXPRESSION IN ENDOMETRIAL HEC-1-B CELLS - AN EXPORT-DEPENDENT MECHANISM OF ACTION", Cancer research, 55(20), 1995, pp. 4729-4738

Abstract

The human endometrial adenocarcinoma HEC-1-B cell line was transfected with an expression vector harboring the human basic fibroblast growth factor (bFGF) cDNA under the control of the human beta-actin gene promoter. Stable transfectants were obtained in which a constitutive, limited overexpression of M(r) 24,000, 22,000, and 18,000 bFGF isoforms was observed. When transfectants were screened for the capacity to release the growth factor, significant amounts of bFGF were present in the conditioned medium and extracellular matrix of the bPGF-B9 clone butnot of the bFGF-AB clone, even though both cell lines produced similar levels of intracellular bFGF. When compared to parental cells, bFGF-B9 cells showed down-regulation of tyrosine kinase fibroblast growth factor receptors along with up-regulation of urokinase-type plasminogenactivator expression which was abolished by incubation of the cell cultures with neutralizing anti-bFGF antibody. In vivo, bFGF-B9 cells formed highly vascularized tumors growing faster than parental cells when injected s.c. in nude mice. Also, they were more potent than nontransfected cells in inducing an angiogenic response in the rabbit cornea assay. In contrast, the bFGF-A8 cell phenotype was indistinguishable from parental cells both in vitro and in vivo. In conclusion, clonal differences exist within the HEC-1-B cell line in the capacity to release bFGF. bFGF export by human endometrial adenocarcinoma cells results in autocrine and paracrine effects that confer a growth advantage in vivo associated with increased neovascularization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 11:13:27