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Titolo:
FUNDULUS-HETEROCLITUS VITELLOGENIN - THE DEDUCED PRIMARY STRUCTURE OFA PISCINE PRECURSOR TO NONCRYSTALLINE, LIQUID-PHASE YOLK PROTEIN
Autore:
LAFLEUR GJ; BYRNE BM; KANUNGO J; NELSON LD; GREENBERG RM; WALLACE RA;
Indirizzi:
UNIV FLORIDA,WHITNEY LAB,9505 OCEAN SHORE BLVD MARINELAND FL 32086 UNIV FLORIDA,COLL MED,DEPT ANAT & CELL BIOL GAINESVILLE FL 32610 MED COLL GEORGIA,INST MOLEC MED & GENET AUGUSTA GA 30912
Titolo Testata:
Journal of molecular evolution
fascicolo: 4, volume: 41, anno: 1995,
pagine: 505 - 521
SICI:
0022-2844(1995)41:4<505:FV-TDP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS-LAEVIS; OOCYTE GROWTH; NUCLEOTIDE-SEQUENCE; MESSENGER-RNA; GENE; PHOSVITIN; LIPOVITELLIN; VERTEBRATE; EXPRESSION; MATURATION;
Keywords:
VITELLOGENIN; VITELLOGENESIS; PHOSVITIN; LIPOVITELLIN; YOLK; TRINUCLEOTIDE REPEAT; TELEOST; FUNDULUS HETEROCLITUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
97
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Lafleur et al., "FUNDULUS-HETEROCLITUS VITELLOGENIN - THE DEDUCED PRIMARY STRUCTURE OFA PISCINE PRECURSOR TO NONCRYSTALLINE, LIQUID-PHASE YOLK PROTEIN", Journal of molecular evolution, 41(4), 1995, pp. 505-521

Abstract

We have cloned and sequenced a cDNA encoding a vitellogenin (Vtg) from the mummichog, Fundulus heteroclitus, an estuarine teleost. We constructed a liver cDNA library against RNA from estrogen-treated male mummichogs. Five overlapping cDNA clones totalling 5,197 bp were isolatedthrough a combination of degenerate oligonucleotide probing of the library and PCR. The cDNA sequence contains a 5,112 bp open reading frame. The predicted primary structure of the deduced 1,704-amino-acid protein is 30-40% identical to other documented chordate Vtgs, establishing this Vtg as a member of the ancient Vtg gene family. Of the previously reported chordate Vtg sequences (Xenopus laevis, Gallus domesticus, Ichthyomyzon unicuspis, and Acipenser transmontanus), all four act as precursor proteins to a yolk which is eventually rendered insoluble under physiological conditions, either as crystalline platelets or as noncrystalline granules. The yolk of F. heteroclitus, on the other hand, remains in a soluble state throughout oocyte growth. The putative F. heteroclitus Vtg contains a polyserine region with a relative serinecomposition that is 10-20% higher than that observed for the other Vtgs. The trinucleotide repeats encoding the characteristic polyserine tracts of the phosvitin region follow a previously reported trend: TCX codons on the 5' end and AGY codons toward the 3' end. Whether the difference in Vtg primary structure between F. heteroclitus and that of other chordates is responsible for the differences in yolk structure remains to be elucidated. As the first complete teleost Vtg to be reported, these data will aid in designing nucleotide and immunological probes for detecting Vtg as a reproductive status indicator in F. heteroclitus and other piscine species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:01:30