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Titolo:
IMPACT OF AGE AND ENVIRONMENT ON SOMATIC MUTATION AT THE HPRT GENE OFT-LYMPHOCYTES IN HUMANS
Autore:
JONES IM; THOMAS CB; TUCKER B; THOMPSON CL; PLESHANOV P; VOROBTSOVA I; MOORE DH;
Indirizzi:
LAWRENCE LIVERMORE NATL LAB,BIOL & BIOTECHNOL RES PROGRAM,L-452,POB 808 LIVERMORE CA 94551 NIEHS,DIV BIOMETRY & RISK ASSESSMENT RES TRIANGLE PK NC 27709 MOSCOW BIOL ACT SUBST INST MOSCOW RUSSIA CENT INST ROENTGENOL & RADIOBIOL ST PETERSBURG RUSSIA CALIF PACIFIC MED CTR SAN FRANCISCO CA 94110
Titolo Testata:
Mutation research. DNAging
fascicolo: 1-6, volume: 338, anno: 1995,
pagine: 129 - 139
SICI:
0921-8734(1995)338:1-6<129:IOAAEO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MUTANT FREQUENCY; MOLECULAR ANALYSES; CLONING ASSAY; HUMAN-CELLS; INVIVO; POPULATIONS; DELETIONS; QUANTIFY; SUBSETS;
Keywords:
SOMATIC MUTATION; AGE; HPRT; HUMAN T-LYMPHOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
I.M. Jones et al., "IMPACT OF AGE AND ENVIRONMENT ON SOMATIC MUTATION AT THE HPRT GENE OFT-LYMPHOCYTES IN HUMANS", Mutation research. DNAging, 338(1-6), 1995, pp. 129-139

Abstract

Analysis of two human populations for dependence of somatic mutation on age has revealed both similarities and differences. The studies performed employed peripheral blood lymphocytes and measured the efficiency with which these cells form clones in vitro (cloning efficiency, CE) and the frequency of cells with inactivating mutations of the hypoxanthine phosphoribosyltransferase gene (mutant frequency, MF). The people studied were between 19 and 64 years of age. In one population, composed of 78 never smokers and 140 current smokers from the United States (US), both CE and MF were dependent on age: CE declined with age(p = 0.005); MF increased 0.15 per 10(6) cells per year of age for nonsmokers (p < 0.001) and at 1.3 times that rate for smokers (p = 0.01). Inthe second population, 80 people of unknown smoking status from Russia, the increase in MF per year was even greater, 2.5 times that of theUS nonsmokers (p = 0.001) but the dependence of CE on age was the same as for the US population (p = 0.043). Because the increase of MF of the Russians with age is 2-fold greater than that of the US smokers, the intensity of smoking and/or other environmental exposures, or the susceptibility to these exposures, must account for the difference in age dependent MF increase, not the proportion of Russians that are smokers. Differences in the lymphocyte subpopulations that survived the longer transit from Russia may have contributed to the observed differences in MF. However, overall, the mutant frequency results suggest thatthe Russians were chronically exposed to higher levels of agents thatinduce somatic mutation and that, on an age adjusted basis, the Russia population studied is at increased risk for health consequences fromaccumulated genetic damage.

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Documento generato il 02/12/20 alle ore 05:06:23