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Titolo:
SPLICING VARIANTS OF THE HUMAN GROWTH-HORMONE MESSENGER-RNA - DETECTION IN PITUITARY, MONONUCLEAR-CELLS AND DERMAL FIBROBLASTS
Autore:
PALMETSHOFER A; ZECHNER D; LUGER TA; BARTA A;
Indirizzi:
UNIV VIENNA,VIENNA BIOCTR,INST BIOCHEM,DR BOHRGASSE 9-3 A-1030 VIENNAAUSTRIA UNIV VIENNA,VIENNA BIOCTR,INST BIOCHEM A-1030 VIENNA AUSTRIA UNIV MUNSTER,DEPT DERMATOL,LUDWIG BOLTZMANN INST CELL BIOL & IMMUNOBIOL SKIN MUNSTER GERMANY
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 2, volume: 113, anno: 1995,
pagine: 225 - 234
SICI:
0303-7207(1995)113:2<225:SVOTHG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HUMAN-PLACENTA; MESSENGER-RNA; GENE; PROLACTIN; EXPRESSION; RECEPTOR; DNA; IDENTIFICATION; TRANSCRIPTS;
Keywords:
HUMAN GROWTH HORMONE; CHORIONIC SOMATOMAMMOTROPIN; ALTERNATIVE SPLICING; MONONUCLEAR CELL; DERMAL FIBROBLAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
A. Palmetshofer et al., "SPLICING VARIANTS OF THE HUMAN GROWTH-HORMONE MESSENGER-RNA - DETECTION IN PITUITARY, MONONUCLEAR-CELLS AND DERMAL FIBROBLASTS", Molecular and cellular endocrinology, 113(2), 1995, pp. 225-234

Abstract

The human growth hormone/human chorionic somatomammotropin (hGH/hCS) gene cluster contains five genes: hGH-N, hGH-V, hCS-A, hCS-B, and hCS-L. In this study, the nature of splicing products of their primary transcripts (except hCS-L) was analyzed by nuclease mapping as well as byreverse transcription-polymerase chain reaction (RT-PCR) experiments. All the previously described hGH-N mRNAs encoding the normal 22-K growth hormone, the 20-K variant as well as a transcript lacking the third exon were found in pituitary tissue and in transiently transfected human 293-S eels. In addition, splicing products lacking either exons 3and 4 or exons 2, 3 and 4 were found in both tissues. In accordance to previously reported data,the hGH-V, the hCS-A and the hCS-B genes which are expressed in placental tissue give rise to the 22-K mRNA but not to 20-K mRNA. Furthermore, no hCS mRNA arising from skipping of exon 3 was present, whereas mRNAs arising from ligation of exon 2 to exon5 and of exon 1 to exon 5 were clearly detectable. The various hGH cDNAs were expressed in vivo and screened for lactogenic activity. Only the 22-K and the 20-K variant were active in this assay. All of the hGH-N-derived differentially processed RNAs were found in cell lines of lymphoid (Hut-78) and of myelomonocytic type (U937), which had been recently described to secrete growth hormone. Interestingly, RT-PCR analysis allowed the determination of hGH-N transcripts in dermal fibroblasts. This finding underlines the importance of growth hormone in influencing immune system development and further suggests possible autocrine/paracrine regulatory loops in skin tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:56:36