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Titolo:
NEUROFILAMENT PHOSPHORYLATION AND [I-125] CALMODULIN-BINDING BY CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE IN THE BRAIN SUBCELLULAR-FRACTIONSOF DIISOPROPYL PHOSPHOROFLUORIDATE (DFP)-TREATED HEN/
Autore:
GUPTA RP; ABOUDONIA MB;
Indirizzi:
DUKE UNIV,MED CTR,DEPT PHARMACOL,POB 3813 DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT PHARMACOL DURHAM NC 27710
Titolo Testata:
Neurochemical research
fascicolo: 9, volume: 20, anno: 1995,
pagine: 1095 - 1105
SICI:
0364-3190(1995)20:9<1095:NPA[CB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED DELAYED NEUROTOXICITY; ORTHO-CRESYL PHOSPHATE; CYTOSKELETAL PROTEINS; CDC2 KINASE; CALMODULIN; INVITRO; AUTOPHOSPHORYLATION; PURIFICATION; ISCHEMIA; SUBUNIT;
Keywords:
NEUROFILAMENT PHOSPHORYLATION; CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE; CALMODULIN-BINDING; HEN; DIISOPROPYL PHOSPHOROFLUORIDATE; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R.P. Gupta e M.B. Aboudonia, "NEUROFILAMENT PHOSPHORYLATION AND [I-125] CALMODULIN-BINDING BY CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE IN THE BRAIN SUBCELLULAR-FRACTIONSOF DIISOPROPYL PHOSPHOROFLUORIDATE (DFP)-TREATED HEN/", Neurochemical research, 20(9), 1995, pp. 1095-1105

Abstract

Diisopropyl phosphorofluoridate (DFP) produces organophosphorus ester-induced delayed neurotoxicity (OPIDN) in humans and sensitive animal species, e.g., adult chicken. The chickens were sacrificed 18 days after a single dose of DFP (1.7 mg/kg, sc.), which produced severe ataxiaor paralysis in 10-14 days, We studied Ca2+/calmodulin-dependent in vitro neurofilament phosphorylation by the brain subcellular fractions of control and DFP-treated hens. There was enhanced phosphorylation ofall three NF subunits by the brain supernatant of treated hens. This was accompanied by enhanced autophosphorylation of both Ca2+/CaM-dependent protein kinase II (CaM-kinase II) subunits and increased calmodulin binding using either I-125-CaM or biotinylated calmodulin to only asubunit without concomitant increase in the amount of this enzyme. This enhanced phosphorylation of neurofilament subunits was completely and partially inhibited by mastoparan and KN-62, respectively. There was no alteration in the distribution of CaM-kinase II activity in treated hens and the activity was not related to its concentration in different subcellular fractions. The difference in I-125-CaM binding to CaM-kinase II a subunit in the brain supernatants of control and DFP-treated hens was not altered by its phosphorylation or dephosphorylation. The increased CaM-kinase II activity in the soluble fraction of DFP-treated hen brain may be involved in the aberrant phosphorylation of axonal neurofilaments, and thus play a role in OPIDN.

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Documento generato il 20/09/20 alle ore 09:34:07