Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIRECT OBSERVATION OF MICROCIRCULATION OF THE BASAL REGION OF RAT GASTRIC-MUCOSA
Autore:
OHNO T; KATORI M; NISHIYAMA K; SAIGENJI K;
Indirizzi:
KITASATO UNIV,SCH MED,DEPT PHARMACOL,1-15-1 KITASATO SAGAMIHARA KANAGAWA 228 JAPAN
Titolo Testata:
Journal of gastroenterology
fascicolo: 5, volume: 30, anno: 1995,
pagine: 557 - 564
SICI:
0944-1174(1995)30:5<557:DOOMOT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Keywords:
INTRAVITAL MICROSCOPY; GASTRIC MUCOSAL MICROCIRCULATION; BASAL PART OF THE GASTRIC MUCOSA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
T. Ohno et al., "DIRECT OBSERVATION OF MICROCIRCULATION OF THE BASAL REGION OF RAT GASTRIC-MUCOSA", Journal of gastroenterology, 30(5), 1995, pp. 557-564

Abstract

We modified and improved techniques for the intravital microscopic observation of the rat gastric microcirculation. The stomach of anesthetized rats was cut along the greater curvature, and the posterior wall of the glandular stomach was fixed in a chamber with the serosal side up and perfused with warmed Tyrode's solution. A portion of the muscularis externa was resected with the serosa to make an observation window. Vascular casts were studied histologically after the injection of Monastral blue B gelatin solution. Vascular casts revealed that most ofthe microvasculature observed in the window was not located in the submucosa, but in the basal part of the mucosa. Microscopic observation showed that the basal mucosal arterioles branched to form the mucosal capillaries, and the collecting venules from the mucosal surface were seen in cross-sections to drain into the venules located in the basal mucosa, without penetrating the muscularis mucosae. Topical application of acetylcholine (0.03-10 mu M) to the window dilated the arterioles, and topical application of epinephrine (0.03-3 mu M) constricted them dose-dependently without affecting the collecting venules and the venules. This method made possible the direct observation of the microvasculature in the basal mucosa of the stomach, in which common microvessel characteristics were shown.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:59:26