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Titolo:
DEMONSTRATION OF KAPPA(3)-OPIOID RECEPTORS IN THE SH-SY5Y HUMAN NEUROBLASTOMA CELL-LINE
Autore:
CHENG J; STANDIFER KM; TUBLIN PR; SU W; PASTERNAK GW;
Indirizzi:
MEM SLOAN KETTERING CANC CTR,DEPT NEUROL,COTZIAS LAB NEUROONCOL,1275 YORK AVE NEW YORK NY 10021 MEM SLOAN KETTERING CANC CTR,DEPT NEUROL,COTZIAS LAB NEUROONCOL NEW YORK NY 10021 CORNELL UNIV,COLL MED,DEPT NEUROL NEW YORK NY 00000 CORNELL UNIV,COLL MED,DEPT NEUROSCI & PHARMACOL NEW YORK NY 00000
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 65, anno: 1995,
pagine: 170 - 175
SICI:
0022-3042(1995)65:1<170:DOKRIT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MU-OPIOID RECEPTOR; IRREVERSIBLE OPIATE AGONISTS; ADENYLATE-CYCLASE ACTIVITY; NEURO-BLASTOMA CELLS; NALOXONE BENZOYLHYDRAZONE; BINDING; MEMBRANES; EXPRESSION; INHIBITION; INTACT;
Keywords:
MORPHINE; NALOXONE BENZOYLHYDRAZONE; TISSUE CULTURE; DIFFERENTIATION; MU RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
J. Cheng et al., "DEMONSTRATION OF KAPPA(3)-OPIOID RECEPTORS IN THE SH-SY5Y HUMAN NEUROBLASTOMA CELL-LINE", Journal of neurochemistry, 65(1), 1995, pp. 170-175

Abstract

In addition to the mu- and delta-opioid receptors previously reported, the SH-SY5Y human neuroblastoma cell line has high levels of kappa(3) receptors, accounting for 40% of total opioid binding, as measured with [H-3]-diprenorphine binding. Competition studies reveal binding profiles for all three receptor classes that are similar to those observed in brain membranes. Differentiation with retinoic acid increases the levels of opioid receptor binding in the cell line, with the largestelevations in kappa(3) binding. Fully 75% of the increased binding corresponds to kappa(3) sites, which represent 50% of total opioid receptor binding in differentiated cells. Morphine inhibits forskolin-stimulated cyclic AMP accumulation, and this effect is readily blocked by the mu antagonist D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2 (CTAP). Naloxone benzoylhydrazone, a kappa(3) agonist, inhibits forskolin-stimulated cyclic AMP accumulation more potently than morphine and is not reversed by CTAP. These studies indicate that SH-SY5Y cells contain high levels of functional kappa(3) receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 05:24:14