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Titolo:
INHIBITION OF CYTOTOXIC T-LYMPHOCYTE-TRIGGERED APOPTOSIS BY TARGET-CELL SURFACE-COUPLED APROTININ
Autore:
WAGNER L; AVERY RK; BENSINGER L; KUSINITZ F; HIBBERD PL; PASTERNACK MS;
Indirizzi:
MASSACHUSETTS GEN HOSP EAST,INFECT DIS LAB,5TH FLOOR,149 13TH ST BOSTON MA 02129 UNIV VIENNA,DEPT ENDOCRINOL & METAB,INNERE MED KLIN 3 VIENNA AUSTRIA MASSACHUSETTS GEN HOSP,CHILDRENS SERV,INFECT DIS UNIT BOSTON MA 02114 MASSACHUSETTS GEN HOSP,MED SERV,INFECT DIS UNIT BOSTON MA 02114 HARVARD UNIV,SCH MED,DEPT PEDIAT BOSTON MA 02115
Titolo Testata:
Molecular immunology
fascicolo: 12, volume: 32, anno: 1995,
pagine: 853 - 864
SICI:
0161-5890(1995)32:12<853:IOCTAB>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRANZYME-A; DNA FRAGMENTATION; CYTO-TOXICITY; PROTEASES; BREAKDOWN; LEUKEMIA; PROTEIN; ATP;
Keywords:
CYTOTOXIC T-LYMPHOCYTE; CELL-MEDIATED CYTOTOXICITY; PROTEASE INHIBITION; APROTININ; GRANZYME A; APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
L. Wagner et al., "INHIBITION OF CYTOTOXIC T-LYMPHOCYTE-TRIGGERED APOPTOSIS BY TARGET-CELL SURFACE-COUPLED APROTININ", Molecular immunology, 32(12), 1995, pp. 853-864

Abstract

A variety of recent investigations have implicated granzymes A and/orB in the target cell nuclear injury which accompanies cytotoxic T-lymphocyte-mediated cytolysis. Since soluble antiproteases have had limited efficacy in inhibiting CTL-mediated lysis, we developed a method tocouple aprotinin, a peptide inhibitor of serine proteases, to the surface of target cells. Aprotinin modified by N-succinimidyl 3-(2-pyridyldithio)propionate retained trypsin-inhibitory activity, and target cells modified with aprotinin had demonstrable cell surface trypsin-inhibitory activity. Flow cytometry demonstrated that aprotinin was detectable on the target cell surface but underwent modulation at a rather rapid rate. When radiolabeled, aprotinin-coupled target cells were studied in 1-2 hr CTL assays, Cr-51 release was little affected, but (125)IUdR release was reduced up to 75% compared to controls. Correspondingapoptosis analysed by agarose gel electrophoresis and direct cytologic visualization was similarly reduced. Thus, aprotinin bound to the surface of target cells selectively protected target cells against CTL-mediated nuclear injury, and may serve as a model for the development of novel inhibitors of CTL-mediated lysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 17:31:47