Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SALT-DEPENDENT STRUCTURAL-CHANGES OF CHROMATIN IN ISOLATED CHICKEN LIVER NUCLEI AS VISUALIZED BY SCANNING ELECTRON-MICROSCOPY
Autore:
ARAI S; HAYASHI M; NAKANISHI YH;
Indirizzi:
RAKUNO GAKUEN UNIV,DEPT VET RADIOL EBETSU HOKKAIDO 069 JAPAN
Titolo Testata:
Journal of Electron Microscopy
fascicolo: 4, volume: 44, anno: 1995,
pagine: 191 - 197
SICI:
0022-0744(1995)44:4<191:SSOCII>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERPHASE NUCLEI; CELL-NUCLEUS; ORGANIZATION; MATRIX; MODEL; DNA;
Keywords:
ISOLATED NUCLEI; CHICKEN LIVER; CHROMATIN ORGANIZATION; CONDENSED CHROMATIN; SCANNING ELECTRON MICROSCOPY (SEM);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
S. Arai et al., "SALT-DEPENDENT STRUCTURAL-CHANGES OF CHROMATIN IN ISOLATED CHICKEN LIVER NUCLEI AS VISUALIZED BY SCANNING ELECTRON-MICROSCOPY", Journal of Electron Microscopy, 44(4), 1995, pp. 191-197

Abstract

MgCl2- and KCl-dependent structural changes of chromatin in isolated chicken liver nuclei were examined by scanning electron microscopy. Inthe absence of salts, the chromatin appeared as granular or knobby fibrillar structures, 15-30 nm in diameter, At 0.2 mM MgCl2, the structures condensed into 30-60 nm fibers. At 2 mM MgCl2, most chromatin was present as condensed chromatin masses, Above 50 mM MgCl2, the condensed chromatin masses were loosened. At KCl concentrations higher than 25mM, the chromatin appeared as discrete 30-60 nm fibers. However, no condensed chromatin masses were formed at any of the KCl concentrationsexamined in this study. These results indicate that the MgCl2- or KCl-dependent transitions from a lower to a higher level of chromatin organization in the isolated nuclei are comparable to those reported in isolated chromatin fragments, and that KCl is ineffective in the formation of condensed chromatin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 03:47:54