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Titolo:
LEISHMANIA (VIANNIA) LAINSONI OCCUPIES A UNIQUE NICHE WITHIN THE SUBGENUS VIANNIA
Autore:
FRESH S; DEBRUIJN MHL; MENDOZALEON JA; BARKER DC;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,MOLTENO LABS,NATL INST MED RES,MRC OUTSTN,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND UNIV CAMBRIDGE,DEPT PATHOL,MOLTENO LABS,NATL INST MED RES,MRC OUTSTN CAMBRIDGE CB2 1QP ENGLAND
Titolo Testata:
Transactions of the Royal Society of Tropical Medicine and Hygiene
fascicolo: 2, volume: 89, anno: 1995,
pagine: 231 - 236
SICI:
0035-9203(1995)89:2<231:L(LOAU>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTZOMYIA-UBIQUITALIS PSYCHODIDAE; KINETOPLAST DNA MINICIRCLES; CUTANEOUS LEISHMANIASIS; PARA STATE; SP-N; AMAZONIAN BRAZIL; IDENTIFICATION; DIAGNOSIS; AMPLIFICATION; HYBRIDIZATION;
Keywords:
LEISHMANIA LAINSONI; CHARACTERIZATION; NUCLEAR DNA; KDNA; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S. Fresh et al., "LEISHMANIA (VIANNIA) LAINSONI OCCUPIES A UNIQUE NICHE WITHIN THE SUBGENUS VIANNIA", Transactions of the Royal Society of Tropical Medicine and Hygiene, 89(2), 1995, pp. 231-236

Abstract

Leishmania lainsoni has recently been recognized as a new peripylarian species belonging to the subgenus Viannia and the L. braziliensis complex. It has been isolated from its sandfly vector, reservoir host and cutaneous lesions of human patients. Microscopical examination has shown characteristics which are different from those of other members of the L. braziliensis complex. Nuclear deoxyribonucleic acid (DNA) hybridization patterns with a beta tubulin probe and kinetoplast DNA buoyant density measurements show close similarities with other species ofthe L. braziliensis complex. However, kinetoplast DNA restriction enzyme fragment patterns of L. (V.) lainsoni isolates show similarities to L. mexicana complex species as well as weak cross hybridization. L. (V.) lainsoni is also amplified with L. braziliensis complex specific polymerase chain reaction (PCR) primers but it requires a lower annealing temperature and gives a 300 base pair PCR product. A possible model for the binding of PCR primers to the L. (V.) lainsoni kinetoplast DNA minicircle is proposed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:07:12