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Titolo:
RADIOLYSIS OF RADIOIMMUNOCONJUGATES - REDUCTION IN ANTIGEN-BINDING ABILITY BY ALPHA-PARTICLE RADIATION
Autore:
LARSEN RH; BRULAND OS;
Indirizzi:
UNIV OSLO,DEPT CHEM,POB 1033 N-0315 OSLO NORWAY
Titolo Testata:
Journal of labelled compounds & radiopharmaceuticals
fascicolo: 10, volume: 36, anno: 1995,
pagine: 1009 - 1018
SICI:
0362-4803(1995)36:10<1009:ROR-RI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL LYMPHOMA; MONOCLONAL-ANTIBODIES; OSTEO-SARCOMA; RADIOIMMUNOTHERAPY; RADIOBIOLOGY; RADIOTHERAPY; PROTEINS;
Keywords:
RADIOLYSIS; ALPHA-PARTICLES; RADIOIMMUNOCONJUGATES; ANTIGEN-BINDING ABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
R.H. Larsen e O.S. Bruland, "RADIOLYSIS OF RADIOIMMUNOCONJUGATES - REDUCTION IN ANTIGEN-BINDING ABILITY BY ALPHA-PARTICLE RADIATION", Journal of labelled compounds & radiopharmaceuticals, 36(10), 1995, pp. 1009-1018

Abstract

The potential of delivering a high and localized radiation dose usingalpha-particle-emitting radioimmunoconjugates (RIC's) is investigatedas treatment for some types of cancer. High specific activity of the RIC may for some uses be desirable, allowing enrichment of the radionuclide on membrane antigens on target cells. Radiation exposure during the radiolabelling procedures may, however, reduce the antigen bindingability of RIC's of high specific activity. The influence of alpha-particle dose on the cell binding fraction was therefore investigated for two monoclonal antibodies, i.e., TP-1 F(ab')(2) and TP-3 IgG. Samples of I-125-labelled MoAb were added different amounts of [At-211]astatide to give accumulated doses ranging from 50 to 50,000 Gy. After the At-211 had decayed 15 half-lives, the cell binding fractions were measured. The results show that [At-211] astatide activities up to 25 kBq/mu l, corresponding to an accumulated alpha-particle radiation dose ofapproximately 1,000 Gy, could be tolerated without significantly reducing the cell binding fraction, whereas higher doses gradually reducedthe immunoreactivity. Although there may be differences in dose sensitivity of different radioimmunoconjugate due to differences in radionuclide conjugation methods, size of the antibody and structure of the antigen binding portion, the results presented here indicate that it ispossible to produce radioimmunoconjugates of high specific activity with currently used methods of preparation. The potential problem of radiolysis should, however, be taken into consideration when planning procedures for preparation and use of alpha-particle-emitting radioimmunoconjugates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:11:09