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Titolo:
CONDITIONS FOR ENGRAFTMENT OF HUMAN ACUTE MYELOID-LEUKEMIA (AML) IN SCID MICE
Autore:
TERPSTRA W; PRINS A; VISSER T; WOGNUM B; WAGEMAKER G; LOWENBERG B; WIELENGA J;
Indirizzi:
UNIV HOSP DIJKZIGT,DEPT HEMATOL,DR MOLEWATERPL 40 3015 GD ROTTERDAM NETHERLANDS ERASMUS UNIV ROTTERDAM,INST HEMATOL ROTTERDAM NETHERLANDS
Titolo Testata:
Leukemia
fascicolo: 9, volume: 9, anno: 1995,
pagine: 1573 - 1577
SICI:
0887-6924(1995)9:9<1573:CFEOHA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE; CELLS; GROWTH; MODEL;
Keywords:
ACUTE MYELOID LEUKEMIA; SCID MOUSE; CHIMERA; INTERLEUKIN-3; IRRADIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
W. Terpstra et al., "CONDITIONS FOR ENGRAFTMENT OF HUMAN ACUTE MYELOID-LEUKEMIA (AML) IN SCID MICE", Leukemia, 9(9), 1995, pp. 1573-1577

Abstract

Transplantation of human AML into severe combined immuno-deficient (SCID) mice provides a useful experimental model but graft failures havebeen reported. We investigated the influence of a number of factors on the outgrowth of AML in the SCID mouse bone marrow (BM). The transplantation route and total body irradiation (TBI) were examined using the cell line HL-60 as a model for AML. The role of graft size and recombinant human IL-3 (IL-3) were investigated with patient samples of AMLcells. Intravenous transplantation was demonstrated to be superior tointraperitoneal transplantation. Pretransplant conditioning resulted in a dose-dependent increase of AML growth in the SCID mouse. Cell dose titrations ranging from 3 x 10(7)-3.6 x 10(5) AML cells i.v. per mouse revealed a minimum of 1.1x10(6) required for reproducible engraftment. Earlier and more extensive infiltration by human AML cells was seen following injection of greater cell numbers. IL-3 given post-transplantation SCID mouse recipients, promoted AML growth in three cases, whereas a fourth AML cell specimen also grew without support of IL-3. Invitro growth factor responsiveness of AML cells to IL-3 did not predict IL-3 dependence of AML growth in vivo.

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Documento generato il 30/11/20 alle ore 06:34:25