Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANALYSIS OF THE MOTILITY PARAMETERS OF IN-VITRO HYPERACTIVATED HAMSTER SPERMATOZOA
Autore:
SHIVAJI S; PEEDICAYIL J; DEVI LG;
Indirizzi:
CTR CELLULAR & MOLEC BIOL,UPPAL RD HYDERABAD 500007 ANDHRA PRADESH INDIA
Titolo Testata:
Molecular reproduction and development
fascicolo: 2, volume: 42, anno: 1995,
pagine: 233 - 247
SICI:
1040-452X(1995)42:2<233:AOTMPO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM ACROSOME REACTION; GUINEA-PIG SPERMATOZOA; MOVEMENT CHARACTERISTICS; RAT SPERM; CYCLIC-AMP; INVITRO FERTILIZATION; CAPACITATION INVITRO; RABBIT SPERMATOZOA; CUMULUS-OOPHORUS; PLASMA-MEMBRANE;
Keywords:
HYPERACTIVATED SPERMATOZOA; MOTILITY PATTERNS; MOTILITY PARAMETERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
73
Recensione:
Indirizzi per estratti:
Citazione:
S. Shivaji et al., "ANALYSIS OF THE MOTILITY PARAMETERS OF IN-VITRO HYPERACTIVATED HAMSTER SPERMATOZOA", Molecular reproduction and development, 42(2), 1995, pp. 233-247

Abstract

Golden hamster cauda epididymal spermatozoa under in vitro capacitating conditions exhibited time-dependent transformation of motility pattern, and the frequency of occurrence of a particular motility type wasfound to be dependent on the depth of the motility chamber used. The nonhyperactivated spermatozoa with planar motility were the most predominant at 0 hr irrespective of the depth of the motility chamber. But spermatozoa with the helical motility pattern were not detectable up to 6 hr when the Makler chamber was used, whereas in both the slide chamber and cannula, by 2 hr such spermatozoa constituted 90% of the total spermatozoa. However, by 6 hr the hyperactivated circular moving spermatozoa were the predominant type in all the chambers. Sperm motilitychamber depths were also found to effect the motility parameter values of hamster spermatozoa, but this effect was also found to be dependent on the type of motility. Increase in chamber depth did not alter any of the motility parameter Values of spermatozoa with hatchet type ofmotility and only increased the amplitude of lateral head displacement (ALH) in planar type. But in spermatozoa with the helical type of motility, an increase in chamber depth increased the progressive velocity (VSL), path velocity (VAP), curvilinear velocity (VCL), straightness(STR), linearity (LIN), and ALH. In spermatozoa with the circular type of motility, an increase in VSL, VAP, VCL, and ALH was also observed, but STR and LIN decreased. The hyperactivated spermatozoa could be distinguished from the nonhyperactivated spermatozoa because the formerwere swimming in circles, had low progressive velocity, decreased straight ness and linearity of path, and also exhibited an increase in the amplitude of lateral head displacement compared to the nonhyperactivated spermatozoa. Further, the spermatozoa with helical motility couldbe differentiated from the nonhyperactivated, circular, and hatchet spermatozoa in that they had the highest VSL, VAP, VCL, and ALH. Spermatozoa with hatchet movement were slow and exhibited very low STR and LIN. Thus the motility parameters could be used to distinguish the nonhyperactivated and hyperactivated distal cauda epididymal spermatozoa. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 01:55:30