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Titolo:
AUXIN-BINDING PROTEIN (ABP1) IS NOT CONFINED TO THE OUTER EPIDERMIS OF MAIZE (ZEA-MAYS L) COLEOPTILES
Autore:
KAYSER B; KLAMBT D;
Indirizzi:
UNIV BONN,INST BOT,MECKENHEIMER ALLEE 170 D-53115 BONN GERMANY
Titolo Testata:
Botanica acta
fascicolo: 4, volume: 108, anno: 1995,
pagine: 365 - 373
SICI:
0932-8629(1995)108:4<365:AP(INC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOLOGICAL METHODS; MOLECULAR-CLONING; CORN COLEOPTILES; PLASMA-MEMBRANE; GROWTH; GENE; RECEPTOR; SITES; CELLS; RNA;
Keywords:
ABP1; ABP1-MESSENGER-RNA; AUXIN-BINDING PROTEIN; COLEOPTILE; IN SITU HYBRIDIZATION; MESSENGER-RNA-TITRATION; ZEA MAYS L;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
B. Kayser e D. Klambt, "AUXIN-BINDING PROTEIN (ABP1) IS NOT CONFINED TO THE OUTER EPIDERMIS OF MAIZE (ZEA-MAYS L) COLEOPTILES", Botanica acta, 108(4), 1995, pp. 365-373

Abstract

Maize seedlings were studied for their expression patterns of ABP1-mRNA and ABP1. In situ hybridization did not reveal hot spots of ABP1-mRNA accumulation. This result was supported by northern hybridization. In coleoptiles the ABP1-mRNA remains constant during day 1 to day 5 and is of low abundance (1.3 pg/mu g total RNA). Northern blots indicated that in primary roots the mRNA level is even 10 times lower. NeitherABP1-mRNA nor ABP1 was found to be concentrated within the outer epidermis of the coleoptile. Analysis of immunostained western blots did not reveal pronounced differences in ABP1 content on the basis of equalamounts of fresh weight or total protein. We therefore assume ABP1 tobe more or less equally distributed among the cells of the shoot tissues of maize seedlings.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:42:12