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Titolo:
SELECTIVE RECOVERY OF STREPTOSPORANGIUM FRAGILE FROM SOIL BY INDIRECTIMMUNOMAGNETIC CAPTURE
Autore:
MULLINS PH; GURTLER H; WELLINGTON EMH;
Indirizzi:
UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND NOVO NORDISK AS DK-2880 BAGSVAERD DENMARK
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 9
pagine: 2149 - 2156
SICI:
1350-0872(1995)141:<2149:SROSFF>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; MONOCLONAL-ANTIBODIES; NONSTERILE SOIL; RAPID ISOLATION; LAKE WATER; SEPARATION; SALMONELLA; CELLS; BACTERIA; FOODS;
Keywords:
IMMUNOCAPTURE; STREPTOSPORANGIUM; SOIL; MAGNETIC BEADS; SELECTIVE ISOLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
P.H. Mullins et al., "SELECTIVE RECOVERY OF STREPTOSPORANGIUM FRAGILE FROM SOIL BY INDIRECTIMMUNOMAGNETIC CAPTURE", Microbiology, 141, 1995, pp. 2149-2156

Abstract

A polyclonal antibody raised to Streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of Streptosporangium, as determined by dot immunoblotting. An indirect immunomagnetic capture method was developed forthe recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit M-280 Dynabeads. The effects of different soil blocking agents, antibody labelling concentrations and spore/Dynabead capture times on the recovery of S. fragile spores were investigated. Pre-blocking of antibody binding sites within the soil, with either2% partially hydrolysed gelatin or 10% skimmed milk, was essential prior to immunomagnetic capture, Increasing the capture time from 15 to 60 min did not affect spore recovery; however, a 10-fold decline in the magnetic bead concentration did result in a significantly lower recovery of spores from soil. S, fragile was selectively enriched (1:190-fold) when present as a mixed population with Arthrobacter oxydans in sterile soil. The indirect immunomagnetic capture method was used to selectively recover S. fragile spores seeded into non-sterile soil, although some background binding of non-target bacteria was noted. The target was successfully recovered from a sterile soil microcosm after 14 d incubation and the capture rate was increased by the inclusion of aninitial soil dispersion and biomass concentration procedure, using the ion-exchange resin Chelex 100.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 04:56:54