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Titolo:
SPECIES-SPECIFIC IDENTIFICATION OF MYCOBACTERIUM-BOVIS BY PCR
Autore:
RODRIGUEZ JG; MEJIA GA; DELPORTILLO P; PATARROYO ME; MURILLO LA;
Indirizzi:
UNIV NACL COLOMBIA,HOSP SAN JUAN DIOS,INST INMUNOL,CARRERA 10 1-01 BOGOTA COLOMBIA UNIV NACL COLOMBIA,HOSP SAN JUAN DIOS,INST INMUNOL BOGOTA COLOMBIA
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 9
pagine: 2131 - 2138
SICI:
1350-0872(1995)141:<2131:SIOMBP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEOXYRIBONUCLEIC-ACID RELATEDNESS; RESTRICTION ENDONUCLEASE ANALYSIS; POLYMERASE CHAIN-REACTION; TUBERCULOSIS COMPLEX; ARBITRARY PRIMERS; DNA; AMPLIFICATION; ANTIGEN; BCG; DIAGNOSIS;
Keywords:
RAPD; PCR; DIAGNOSIS; MYCOBACTERIUM BOVIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J.G. Rodriguez et al., "SPECIES-SPECIFIC IDENTIFICATION OF MYCOBACTERIUM-BOVIS BY PCR", Microbiology, 141, 1995, pp. 2131-2138

Abstract

The Random Amplified Polymorphic DNA (RAPD) technique was used in theidentification of a species-specific fragment of Mycobacterium bovis. A fragment of approximately 500 bp was amplified from the genome of 15 different IM. bovis strains, including M. bovis BCG Pasteur, but wasshown to be absent in 26 different mycobacteria and 20 different clinical isolates of Mycobacterium tuberculosis. When the fragment was used as a probe in a Southern blot analysis, several radioactive bands common to M. tuberculosis and M. bovis were observed. However, this fragment hybridized specifically to a 2900 bp EcoRI fragment in the M. bovis genome, but failed to hybridize in either M. tuberculosis or M. avium chromosomal DNA. Based on a partial nucleotide sequence of the 500 bp fragment, two oligonucleotide primers were designed and a PCR assaywas developed. Using purified mycobacterial DNA samples, only M. bovis and M. bovis BCG rendered a unique amplification band. This PCR assay is able to detect down to 10 fg purified M. bovis DNA, which corresponds roughly to two bacilli. The assay is also useful for identifying the bacilli directly from uncultured biological samples, such as milk.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 04:12:02